Telomere terminal G/C strand synthesis: measuring telomerase action and C-rich fill-in.

Yong Zhao, Jerry W. Shay, Woodring E. Wright

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Telomerase is present in most human cancers, and proliferative stem cells including germline cells. Telomerase plays an essential role in tumorigenesis by maintaining/elongating telomeric DNA, and thus preventing the telomere shortening that results in replicative senescence. Understanding telomerase action in vivo has important implication for both cancer and aging, but there are not robust methods for monitoring telomerase action. By combining a series of cell biological and biochemical approaches, and taking advantage of the enzyme DSN that specifically cuts double-stranded DNA and releases the telomeric overhangs, we have developed a method to monitor telomerase action during one cell cycle. Here, we describe this method using HeLa carcinoma cells as an example.

Original languageEnglish (US)
Pages (from-to)63-75
Number of pages13
JournalMethods in molecular biology (Clifton, N.J.)
Volume735
DOIs
StatePublished - 2011

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Telomerase
Telomere
Telomere Shortening
Neoplastic Stem Cells
Cell Aging
DNA
HeLa Cells
Cell Cycle
Carcinogenesis
Carcinoma
Enzymes
Neoplasms

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Telomere terminal G/C strand synthesis : measuring telomerase action and C-rich fill-in. / Zhao, Yong; Shay, Jerry W.; Wright, Woodring E.

In: Methods in molecular biology (Clifton, N.J.), Vol. 735, 2011, p. 63-75.

Research output: Contribution to journalArticle

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