Telomeric length and telomerase activity vary with age in peripheral blood cells obtained from normal individuals

Hiroshi Iwama, Kazuma Ohyashiki, Junko H. Ohyashiki, Shigifumi Hayashi, Naoyuki Yahata, Keiko Ando, Keisuke Toyama, Akinori Hoshika, Masaru Takasaki, Mayumi Mori, Jerry W. Shay

Research output: Contribution to journalArticle

205 Citations (Scopus)

Abstract

The telomerase activity and length of telomeres of peripheral blood mononuclear cells obtained from 124 healthy individuals aged 4-95 years was measured. Telomerase activity level was semiquantitatively assessed by a fluorescent-telomeric repeat amplification protocol (fluorescent-TRAP) using an internal telomerase assay standard, fluorescent primers and an automated laser fluorescent DNA sequencer. Telomeric length, measured by assay of terminal restriction fragments (TRFs), was determined in HinfI-digested DNA by Southern blot analysis using a (TTAGGG)4 probe. TRF length was determined in 80 individuals and age-related progressive reduction of size was observed. TRF length in peripheral blood mononuclear cells obtained from normal individuals (aged 4-39 years) decreased by approximately 84 bp per year, while in individuals aged ≤ 40 years it decreased by 41 bp per year. In contrast, telomerase activity showed an apparent biphasic pattern with aging. Individuals aged 4-39 years showed a progressive decrease in telomerase activity, whereas 65% of those aged ≤ 40 years showed relatively stable but very low telomerase activity, and the remaining individuals aged ≤ 40 years had no detectable telomerase activity. These data obtained from normal individuals might in the future be of value to help risk stratify and manage the care of patients with leukemia.

Original languageEnglish (US)
Pages (from-to)397-402
Number of pages6
JournalHuman Genetics
Volume102
Issue number4
DOIs
StatePublished - 1998

Fingerprint

Telomerase
Blood Cells
DNA
Telomere
Southern Blotting
Patient Care
Leukemia
Lasers

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

Telomeric length and telomerase activity vary with age in peripheral blood cells obtained from normal individuals. / Iwama, Hiroshi; Ohyashiki, Kazuma; Ohyashiki, Junko H.; Hayashi, Shigifumi; Yahata, Naoyuki; Ando, Keiko; Toyama, Keisuke; Hoshika, Akinori; Takasaki, Masaru; Mori, Mayumi; Shay, Jerry W.

In: Human Genetics, Vol. 102, No. 4, 1998, p. 397-402.

Research output: Contribution to journalArticle

Iwama, H, Ohyashiki, K, Ohyashiki, JH, Hayashi, S, Yahata, N, Ando, K, Toyama, K, Hoshika, A, Takasaki, M, Mori, M & Shay, JW 1998, 'Telomeric length and telomerase activity vary with age in peripheral blood cells obtained from normal individuals', Human Genetics, vol. 102, no. 4, pp. 397-402. https://doi.org/10.1007/s004390050711
Iwama, Hiroshi ; Ohyashiki, Kazuma ; Ohyashiki, Junko H. ; Hayashi, Shigifumi ; Yahata, Naoyuki ; Ando, Keiko ; Toyama, Keisuke ; Hoshika, Akinori ; Takasaki, Masaru ; Mori, Mayumi ; Shay, Jerry W. / Telomeric length and telomerase activity vary with age in peripheral blood cells obtained from normal individuals. In: Human Genetics. 1998 ; Vol. 102, No. 4. pp. 397-402.
@article{0c89c7bae3f14e24b1e518cbfd9ba95c,
title = "Telomeric length and telomerase activity vary with age in peripheral blood cells obtained from normal individuals",
abstract = "The telomerase activity and length of telomeres of peripheral blood mononuclear cells obtained from 124 healthy individuals aged 4-95 years was measured. Telomerase activity level was semiquantitatively assessed by a fluorescent-telomeric repeat amplification protocol (fluorescent-TRAP) using an internal telomerase assay standard, fluorescent primers and an automated laser fluorescent DNA sequencer. Telomeric length, measured by assay of terminal restriction fragments (TRFs), was determined in HinfI-digested DNA by Southern blot analysis using a (TTAGGG)4 probe. TRF length was determined in 80 individuals and age-related progressive reduction of size was observed. TRF length in peripheral blood mononuclear cells obtained from normal individuals (aged 4-39 years) decreased by approximately 84 bp per year, while in individuals aged ≤ 40 years it decreased by 41 bp per year. In contrast, telomerase activity showed an apparent biphasic pattern with aging. Individuals aged 4-39 years showed a progressive decrease in telomerase activity, whereas 65{\%} of those aged ≤ 40 years showed relatively stable but very low telomerase activity, and the remaining individuals aged ≤ 40 years had no detectable telomerase activity. These data obtained from normal individuals might in the future be of value to help risk stratify and manage the care of patients with leukemia.",
author = "Hiroshi Iwama and Kazuma Ohyashiki and Ohyashiki, {Junko H.} and Shigifumi Hayashi and Naoyuki Yahata and Keiko Ando and Keisuke Toyama and Akinori Hoshika and Masaru Takasaki and Mayumi Mori and Shay, {Jerry W.}",
year = "1998",
doi = "10.1007/s004390050711",
language = "English (US)",
volume = "102",
pages = "397--402",
journal = "Human Genetics",
issn = "0340-6717",
publisher = "Springer Verlag",
number = "4",

}

TY - JOUR

T1 - Telomeric length and telomerase activity vary with age in peripheral blood cells obtained from normal individuals

AU - Iwama, Hiroshi

AU - Ohyashiki, Kazuma

AU - Ohyashiki, Junko H.

AU - Hayashi, Shigifumi

AU - Yahata, Naoyuki

AU - Ando, Keiko

AU - Toyama, Keisuke

AU - Hoshika, Akinori

AU - Takasaki, Masaru

AU - Mori, Mayumi

AU - Shay, Jerry W.

PY - 1998

Y1 - 1998

N2 - The telomerase activity and length of telomeres of peripheral blood mononuclear cells obtained from 124 healthy individuals aged 4-95 years was measured. Telomerase activity level was semiquantitatively assessed by a fluorescent-telomeric repeat amplification protocol (fluorescent-TRAP) using an internal telomerase assay standard, fluorescent primers and an automated laser fluorescent DNA sequencer. Telomeric length, measured by assay of terminal restriction fragments (TRFs), was determined in HinfI-digested DNA by Southern blot analysis using a (TTAGGG)4 probe. TRF length was determined in 80 individuals and age-related progressive reduction of size was observed. TRF length in peripheral blood mononuclear cells obtained from normal individuals (aged 4-39 years) decreased by approximately 84 bp per year, while in individuals aged ≤ 40 years it decreased by 41 bp per year. In contrast, telomerase activity showed an apparent biphasic pattern with aging. Individuals aged 4-39 years showed a progressive decrease in telomerase activity, whereas 65% of those aged ≤ 40 years showed relatively stable but very low telomerase activity, and the remaining individuals aged ≤ 40 years had no detectable telomerase activity. These data obtained from normal individuals might in the future be of value to help risk stratify and manage the care of patients with leukemia.

AB - The telomerase activity and length of telomeres of peripheral blood mononuclear cells obtained from 124 healthy individuals aged 4-95 years was measured. Telomerase activity level was semiquantitatively assessed by a fluorescent-telomeric repeat amplification protocol (fluorescent-TRAP) using an internal telomerase assay standard, fluorescent primers and an automated laser fluorescent DNA sequencer. Telomeric length, measured by assay of terminal restriction fragments (TRFs), was determined in HinfI-digested DNA by Southern blot analysis using a (TTAGGG)4 probe. TRF length was determined in 80 individuals and age-related progressive reduction of size was observed. TRF length in peripheral blood mononuclear cells obtained from normal individuals (aged 4-39 years) decreased by approximately 84 bp per year, while in individuals aged ≤ 40 years it decreased by 41 bp per year. In contrast, telomerase activity showed an apparent biphasic pattern with aging. Individuals aged 4-39 years showed a progressive decrease in telomerase activity, whereas 65% of those aged ≤ 40 years showed relatively stable but very low telomerase activity, and the remaining individuals aged ≤ 40 years had no detectable telomerase activity. These data obtained from normal individuals might in the future be of value to help risk stratify and manage the care of patients with leukemia.

UR - http://www.scopus.com/inward/record.url?scp=0031922795&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031922795&partnerID=8YFLogxK

U2 - 10.1007/s004390050711

DO - 10.1007/s004390050711

M3 - Article

C2 - 9600234

AN - SCOPUS:0031922795

VL - 102

SP - 397

EP - 402

JO - Human Genetics

JF - Human Genetics

SN - 0340-6717

IS - 4

ER -