Temperate membrane-containing halophilic archaeal virus SNJ1 has a circular dsDNA genome identical to that of plasmid pHH205

Ziqian Zhang; Ying Liu; Shuai Wang; Di Yang; Yichen Cheng; Jiani Hu; Jin Chen; Yunjun Mei; Ping Shen; Dennis H. Bamford; Xiangdong Chen

doi:10.1016/j.virol.2012.05.036

Temperate membrane-containing halophilic archaeal virus SNJ1 has a circular dsDNA genome identical to that of plasmid pHH205

Ziqian Zhang, Ying Liu, Shuai Wang, Di Yang, Yichen Cheng, Jiani Hu, Jin Chen, Yunjun Mei, Ping Shen, Dennis H. Bamford, Xiangdong Chen

Research output: Contribution to journal › Article › peer-review

50 Scopus citations

Abstract

A temperate haloarchaeal virus, SNJ1, was induced from the lysogenic host, Natrinema sp. J7-1, with mitomycin C, and the virus produced plaques on lawns of Natrinema sp. J7-2. Optimization of the induction conditions allowed us to increase the titer from ~10⁴PFU/ml to ~10¹¹PFU/ml. Single-step growth curves exhibited a burst size of ~100PFU/cell. The genome of SNJ1 was observed to be a circular, double-stranded DNA (dsDNA) molecule (16,341bp). Surprisingly, the sequence of SNJ1 was identical to that of a previously described plasmid, pHH205, indicating that this plasmid is the provirus of SNJ1. Several structural protein-encoding genes were identified in the viral genome. In addition, the comparison of putative packaging ATPase sequences from bacterial, archaeal and eukaryotic viruses, as well as the presence of lipid constituents from the host phospholipid pool, strongly suggest that SNJ1 belongs to the PRD1-type lineage of dsDNA viruses, which have an internal membrane.

Original language	English (US)
Pages (from-to)	233-241
Number of pages	9
Journal	Virology
Volume	434
Issue number	2
DOIs	https://doi.org/10.1016/j.virol.2012.05.036
State	Published - Dec 20 2012
Externally published	Yes

Keywords

ATPase
Haloarchaea
Induction
Internal membrane
Lysogenic host
Plasmid
Temperate virus

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2012.05.036

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@article{ce9906f3d07b4c9f882698a035aa2629,

title = "Temperate membrane-containing halophilic archaeal virus SNJ1 has a circular dsDNA genome identical to that of plasmid pHH205",

abstract = "A temperate haloarchaeal virus, SNJ1, was induced from the lysogenic host, Natrinema sp. J7-1, with mitomycin C, and the virus produced plaques on lawns of Natrinema sp. J7-2. Optimization of the induction conditions allowed us to increase the titer from ~104PFU/ml to ~1011PFU/ml. Single-step growth curves exhibited a burst size of ~100PFU/cell. The genome of SNJ1 was observed to be a circular, double-stranded DNA (dsDNA) molecule (16,341bp). Surprisingly, the sequence of SNJ1 was identical to that of a previously described plasmid, pHH205, indicating that this plasmid is the provirus of SNJ1. Several structural protein-encoding genes were identified in the viral genome. In addition, the comparison of putative packaging ATPase sequences from bacterial, archaeal and eukaryotic viruses, as well as the presence of lipid constituents from the host phospholipid pool, strongly suggest that SNJ1 belongs to the PRD1-type lineage of dsDNA viruses, which have an internal membrane.",

keywords = "ATPase, Haloarchaea, Induction, Internal membrane, Lysogenic host, Plasmid, Temperate virus",

author = "Ziqian Zhang and Ying Liu and Shuai Wang and Di Yang and Yichen Cheng and Jiani Hu and Jin Chen and Yunjun Mei and Ping Shen and Bamford, {Dennis H.} and Xiangdong Chen",

note = "Funding Information: This work was supported by grants from the National Basic Research Program of China (973 Program) (no. 2011CB808800 ), the National Natural Science Foundation of China (no. 30870133 ), and the Chinese 111 Project (no. B06018 ). The funding for D.H.B. was obtained from the Academy of Finland, Academy Professor Funding (Grant nos. 256197 and 256518 ). ",

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doi = "10.1016/j.virol.2012.05.036",

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volume = "434",

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T1 - Temperate membrane-containing halophilic archaeal virus SNJ1 has a circular dsDNA genome identical to that of plasmid pHH205

AU - Zhang, Ziqian

AU - Liu, Ying

AU - Wang, Shuai

AU - Yang, Di

AU - Cheng, Yichen

AU - Hu, Jiani

AU - Chen, Jin

AU - Mei, Yunjun

AU - Shen, Ping

AU - Bamford, Dennis H.

AU - Chen, Xiangdong

N1 - Funding Information: This work was supported by grants from the National Basic Research Program of China (973 Program) (no. 2011CB808800 ), the National Natural Science Foundation of China (no. 30870133 ), and the Chinese 111 Project (no. B06018 ). The funding for D.H.B. was obtained from the Academy of Finland, Academy Professor Funding (Grant nos. 256197 and 256518 ).

PY - 2012/12/20

Y1 - 2012/12/20

N2 - A temperate haloarchaeal virus, SNJ1, was induced from the lysogenic host, Natrinema sp. J7-1, with mitomycin C, and the virus produced plaques on lawns of Natrinema sp. J7-2. Optimization of the induction conditions allowed us to increase the titer from ~104PFU/ml to ~1011PFU/ml. Single-step growth curves exhibited a burst size of ~100PFU/cell. The genome of SNJ1 was observed to be a circular, double-stranded DNA (dsDNA) molecule (16,341bp). Surprisingly, the sequence of SNJ1 was identical to that of a previously described plasmid, pHH205, indicating that this plasmid is the provirus of SNJ1. Several structural protein-encoding genes were identified in the viral genome. In addition, the comparison of putative packaging ATPase sequences from bacterial, archaeal and eukaryotic viruses, as well as the presence of lipid constituents from the host phospholipid pool, strongly suggest that SNJ1 belongs to the PRD1-type lineage of dsDNA viruses, which have an internal membrane.

AB - A temperate haloarchaeal virus, SNJ1, was induced from the lysogenic host, Natrinema sp. J7-1, with mitomycin C, and the virus produced plaques on lawns of Natrinema sp. J7-2. Optimization of the induction conditions allowed us to increase the titer from ~104PFU/ml to ~1011PFU/ml. Single-step growth curves exhibited a burst size of ~100PFU/cell. The genome of SNJ1 was observed to be a circular, double-stranded DNA (dsDNA) molecule (16,341bp). Surprisingly, the sequence of SNJ1 was identical to that of a previously described plasmid, pHH205, indicating that this plasmid is the provirus of SNJ1. Several structural protein-encoding genes were identified in the viral genome. In addition, the comparison of putative packaging ATPase sequences from bacterial, archaeal and eukaryotic viruses, as well as the presence of lipid constituents from the host phospholipid pool, strongly suggest that SNJ1 belongs to the PRD1-type lineage of dsDNA viruses, which have an internal membrane.

KW - ATPase

KW - Haloarchaea

KW - Induction

KW - Internal membrane

KW - Lysogenic host

KW - Plasmid

KW - Temperate virus

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ER -

Temperate membrane-containing halophilic archaeal virus SNJ1 has a circular dsDNA genome identical to that of plasmid pHH205

Abstract

Keywords

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