Temperature-dependent cleavage of chromatin by micrococcal nuclease near the nucleosome center

Sue Ying Huang, William T. Garrard

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Digestion of nuclei at 4°C with micrococcal nuclease results in significant intranucleosomal cleavage compared to digestion conducted at 37°C. Employing nucleoprotein gel electrophoresis in one dimension followed by DNA electrophoresis in a second dimension, we demonstrate that such temperature-sensitive, internal cleavage predominantly occurs about 20 bp from the nucleosome center. We suggest that lower temperatures reduce the stability of hydrophobic interactions within the histone octamer and lead to a conformational alteration in nucleosomes that is detected by micrococcal nuclease.

Original languageEnglish (US)
Pages (from-to)89-91
Number of pages3
JournalFEBS Letters
Volume199
Issue number1
DOIs
StatePublished - Apr 7 1986

Fingerprint

Micrococcal Nuclease
Nucleosomes
Electrophoresis
Chromatin
Digestion
Temperature
Nucleoproteins
Hydrophobic and Hydrophilic Interactions
Histones
Gels
DNA

Keywords

  • Chromatin structure
  • Half-nucleosome
  • Histone octamer
  • Micrococcal nuclease
  • Nucleosome
  • Two-dimensional electrophoresis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Temperature-dependent cleavage of chromatin by micrococcal nuclease near the nucleosome center. / Huang, Sue Ying; Garrard, William T.

In: FEBS Letters, Vol. 199, No. 1, 07.04.1986, p. 89-91.

Research output: Contribution to journalArticle

Huang, Sue Ying ; Garrard, William T. / Temperature-dependent cleavage of chromatin by micrococcal nuclease near the nucleosome center. In: FEBS Letters. 1986 ; Vol. 199, No. 1. pp. 89-91.
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