Terminal deoxynucleotidyl transferase-containing cells in peripheral blood: Implications for the surveillance of patients with lymphoblastic leukemia or lymphoma in remission

T. W. Froehlich, G. R. Buchanan, J. A M Cornet, P. A. Sartain, R. G. Smith

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

An indirect immunofluorescence assay was used to quantitate TdT-containing (TdT+) cells in the mononuclear leukocyte fraction of peripheral blood from normal subjects and patients with acute lymphoblastic leukemia (ALL) and lymphoblastic lymphoma (LL). In normal children (10) and adults (10), 0.036% ± 0.014% (mean ±SD) and 0.030% ± 0.015% TdT+ cells were found. In peripheral bloods from 10 children receiving chemotherapy for tumors other than ALL or LL, 0.040% ± 0.039% TdT+ cells were found. Serial determinations were performed on 15 patients with ALL or LL who were in clinical remission. Eight of these patients remained in continuous remission and always had fewer than 0.11% TdT+ cells in their peripheral blood. Three patients who developed systemic relapse were found to have progressively rising numbers of TdT+ cells in their peripheral blood prior to clinical evidence of relapse. All 3 of these patients had >0.1% TdT+ cells in their peripheral blood from 3 to 8 wk prior to clinical relapse. In 3 other patients, localized extramedullary relapse developed, but no trend was found on serial TdT determinations. Thus, the indirect immunofluorescence assay for TdT detects a small population of cells in normal peripheral blood. In patients with ALL, progressive increases above this normal level were associated with subsequent bone marrow relapse.

Original languageEnglish (US)
Pages (from-to)214-220
Number of pages7
JournalBlood
Volume58
Issue number2
StatePublished - Oct 26 1981

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Fingerprint Dive into the research topics of 'Terminal deoxynucleotidyl transferase-containing cells in peripheral blood: Implications for the surveillance of patients with lymphoblastic leukemia or lymphoma in remission'. Together they form a unique fingerprint.

  • Cite this