Tetraethylammonium, a K+ channel blocker, inhibits interferon-γ-induced major histocompatibility class II antigen (Ia) expression and DNA synthesis in rat astrocytes

Kiyoshi Ohira, Bharathi Vayuvegula, Masaru Murakami, Sastry Gollapudi, Elliot Frohman, Stanley van den Noort, Sudhir Gupta

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Astrocytes play an important role in antigen presentation to T lymphocytes by their ability to express major histocompatibility class II (Ia) antigen upon exposure to a number of agents, including interferon-γ (IFN-γ). Astrocytes have been shown to express a variety of voltage-sensitive ion channels including voltage-sensitive K+ channels. The function(s) of these channels in astrocyte functions is not clearly understood. In this investigation, we examined: (1) the comparative effects of mouse, rat, and human recombinant IFN-γ (rIFN-γ) on the induction of Ia antigen and DNA synthesis in rat astrocytes; (2) the effect of tetraethylammonium (TEA), a K+ channel blocker, on rat IFN-γ-induced Ia expression and DNA synthesis in rat astrocytes. Our data show that all INF-γ induce DNA synthesis in rat astrocytes but only rat and mouse and not the human IFN-γ will induce Ia expression. TEA in a dose-dependent manner inhibited both Ia expression and DNA synthesis in rat astrocytes. The concentration kinetics of TEA with regard to maximum inhibition of Ia and DNA synthesis were different. Furthermore, these inhibitory effects were not a result of toxic or nonspecific effect of TEA on astrocytes as demonstrated by viability data and lack of any effect of tetramethylammonium, an analogue of TEA, that does not block K+ ion channels. These data suggest a role of K+ channels in Ia expression and DNA synthesis, therefore in immunological functions of astrocytes.

Original languageEnglish (US)
Pages (from-to)43-49
Number of pages7
JournalJournal of Neuroimmunology
Volume31
Issue number1
DOIs
StatePublished - 1991

Fingerprint

Tetraethylammonium
Histocompatibility Antigens Class II
Astrocytes
Interferons
Antigens
DNA
Ion Channels
Histocompatibility
Poisons
Antigen Presentation
T-Lymphocytes

Keywords

  • Astrocyte
  • DNA synthesis
  • Ia antigen
  • Interferon-γ
  • Potassium channel

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Clinical Neurology
  • Neurology

Cite this

Tetraethylammonium, a K+ channel blocker, inhibits interferon-γ-induced major histocompatibility class II antigen (Ia) expression and DNA synthesis in rat astrocytes. / Ohira, Kiyoshi; Vayuvegula, Bharathi; Murakami, Masaru; Gollapudi, Sastry; Frohman, Elliot; van den Noort, Stanley; Gupta, Sudhir.

In: Journal of Neuroimmunology, Vol. 31, No. 1, 1991, p. 43-49.

Research output: Contribution to journalArticle

Ohira, Kiyoshi ; Vayuvegula, Bharathi ; Murakami, Masaru ; Gollapudi, Sastry ; Frohman, Elliot ; van den Noort, Stanley ; Gupta, Sudhir. / Tetraethylammonium, a K+ channel blocker, inhibits interferon-γ-induced major histocompatibility class II antigen (Ia) expression and DNA synthesis in rat astrocytes. In: Journal of Neuroimmunology. 1991 ; Vol. 31, No. 1. pp. 43-49.
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