Tfb6, a previously unidentified subunit of the general transcription factor TFIIH, facilitates dissociation of Ssl2 helicase after transcription initiation

Kenji Murakami, Brian J. Gibbons, Ralph E. Davis, Shigeki Nagai, Xin Liu, Philip J J Robinson, Tinghe Wu, Craig D. Kaplan, Roger D. Kornberg

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

General transcription factor TFIIH, previously described as a 10-subunit complex, is essential for transcription and DNA repair. An eleventh subunit now identified, termed Tfb6, exhibits 45% sequence similarity to human nuclear mRNA export factor 5. Tfb6 dissociates from TFIIH as a heterodimer with the Ssl2 subunit, a DNA helicase that drives promoter melting for the initiation of transcription. Tfb6 does not, however, dissociate Ssl2 from TFIIH in the context of a fully assembled transcription preinitiation complex. Our findings suggest a dynamic state of Ssl2, allowing its engagement in multiple cellular processes.

Original languageEnglish (US)
Pages (from-to)4816-4821
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number13
DOIs
StatePublished - Mar 27 2012

Keywords

  • DNA repair
  • General transcription factor
  • RNA polymerase II
  • TFIIK
  • Transcription pre-initiation complex
  • Yeast

ASJC Scopus subject areas

  • General

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