TY - JOUR
T1 - TGF-β1 neutralizing antibodies decrease the fibrotic effects of ischemic priapism
AU - Sanli, O.
AU - Armagan, A.
AU - Kandirali, E.
AU - Ozerman, B.
AU - Ahmedov, I.
AU - Solakoglu, S.
AU - Nurten, A.
AU - Tunç, M.
AU - Uysal, V.
AU - Kadioglu, A.
N1 - Funding Information:
*Correspondence: A Kadioglu, Istanbul Faculty of Medicine, Section of Andrology, University of Istanbul, Cerrahi monoblok 1. kat Capa, Istanbul 34790, Turkey. E-mail: onersanli@tnn.net Source of funding: Istanbul University Research Foundation. Received 17 February 2004; revised 21 May 2004; accepted 24 June 2004
PY - 2004/12
Y1 - 2004/12
N2 - The objective of this study was to evaluate the possible role of transforming growth factor beta 1 (TGF-β1) antibodies (ab) for the prevention of fibrotic effects of priapism in a rat model. In total, 30 adult Sprague-Dawley rats were divided into five groups. Priapism with 6 h (group 1), priapism with 6 h + ab (group 2), priapism with 24 h (group 3), priapism with 24 h + ab (group 4) and control (group 5). Priapism was induced with a vacuum erection device and a rubber band was placed at the base of the erect penis. At 1 h after the initiation of priapism, TGF-β1 antibodies were given intracavernosaly. All rats underwent electrical stimulation of the cavernous nerve after 8 weeks. Intracavernous and systemic blood pressures were measured during the procedure. Rats in group 1 showed significantly higher (intracavemosal pressure (ICP) pressures to cavernous nerve stimulation and had higher ICP/BP ratios when compared to other groups. Similarly, histopathologic examination revealed less fibrosis in group 2, compared with the other groups. Consequently, TGF-β1 antibodies antagonise the fibrotic effects of TGF-β1, especially in cases with duration of priapism less than 6 h.
AB - The objective of this study was to evaluate the possible role of transforming growth factor beta 1 (TGF-β1) antibodies (ab) for the prevention of fibrotic effects of priapism in a rat model. In total, 30 adult Sprague-Dawley rats were divided into five groups. Priapism with 6 h (group 1), priapism with 6 h + ab (group 2), priapism with 24 h (group 3), priapism with 24 h + ab (group 4) and control (group 5). Priapism was induced with a vacuum erection device and a rubber band was placed at the base of the erect penis. At 1 h after the initiation of priapism, TGF-β1 antibodies were given intracavernosaly. All rats underwent electrical stimulation of the cavernous nerve after 8 weeks. Intracavernous and systemic blood pressures were measured during the procedure. Rats in group 1 showed significantly higher (intracavemosal pressure (ICP) pressures to cavernous nerve stimulation and had higher ICP/BP ratios when compared to other groups. Similarly, histopathologic examination revealed less fibrosis in group 2, compared with the other groups. Consequently, TGF-β1 antibodies antagonise the fibrotic effects of TGF-β1, especially in cases with duration of priapism less than 6 h.
KW - Animal models
KW - Priapism of penis and clitoris
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U2 - 10.1038/sj.ijir.3901261
DO - 10.1038/sj.ijir.3901261
M3 - Article
C2 - 15284835
AN - SCOPUS:10844231841
SN - 0955-9930
VL - 16
SP - 492
EP - 497
JO - International Journal of Impotence Research
JF - International Journal of Impotence Research
IS - 6
ER -