TGFBR3 loss and consequences in prostate cancer

Nima Sharifi, Elaine M. Hurt, Brian T. Kawasaki, William L. Farrar

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

BACKGROUND. Resistance to transforming growth factor-beta (TGF-β) is important in tumorigenesis. TGF-β resistance mechanisms in prostate cancer are not well understood. METHODS. We have conducted a systematic analysis of TGF-β pathway components with a meta-analysis of seven microarray studies using Oncomine and evaluated the results of TGFBR3 expression in prostate cell lines. Furthermore, we knocked down TGFBR3 in prostate epithelial cells and analyzed the consequences of TGFBR3 knockdown. RESULTS. We found that TGFBR3 is the TGF-β component most commonly downregulated among localized human prostate cancer studies. TGFBR3 knockdown led to focus formation and enhanced expression of CD133, a marker found on prostate cancer stem cells. DNA microarray analysis of TGFBR3 knockdown cells identified 101 genes regulated by TGFBR3. Seven of these genes show a corresponding decrease in clinical prostate cancer specimens, which include genes involved in prostate mass and vasculature. CONCLUSIONS. TGFBR3 downregulation is an important step in prostate tumorigenesis.

Original languageEnglish (US)
Pages (from-to)301-311
Number of pages11
JournalProstate
Volume67
Issue number3
DOIs
StatePublished - Feb 15 2007

Fingerprint

Prostatic Neoplasms
Transforming Growth Factor beta
Prostate
Carcinogenesis
Down-Regulation
Genes
Neoplastic Stem Cells
betaglycan
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Meta-Analysis
Epithelial Cells
Cell Line

Keywords

  • Angiogenesis
  • Betaglycan
  • Prostate cancer
  • Prostate intraepithelial neoplasia
  • Stem cells
  • TGF-β

ASJC Scopus subject areas

  • Urology

Cite this

Sharifi, N., Hurt, E. M., Kawasaki, B. T., & Farrar, W. L. (2007). TGFBR3 loss and consequences in prostate cancer. Prostate, 67(3), 301-311. https://doi.org/10.1002/pros.20526

TGFBR3 loss and consequences in prostate cancer. / Sharifi, Nima; Hurt, Elaine M.; Kawasaki, Brian T.; Farrar, William L.

In: Prostate, Vol. 67, No. 3, 15.02.2007, p. 301-311.

Research output: Contribution to journalArticle

Sharifi, N, Hurt, EM, Kawasaki, BT & Farrar, WL 2007, 'TGFBR3 loss and consequences in prostate cancer', Prostate, vol. 67, no. 3, pp. 301-311. https://doi.org/10.1002/pros.20526
Sharifi N, Hurt EM, Kawasaki BT, Farrar WL. TGFBR3 loss and consequences in prostate cancer. Prostate. 2007 Feb 15;67(3):301-311. https://doi.org/10.1002/pros.20526
Sharifi, Nima ; Hurt, Elaine M. ; Kawasaki, Brian T. ; Farrar, William L. / TGFBR3 loss and consequences in prostate cancer. In: Prostate. 2007 ; Vol. 67, No. 3. pp. 301-311.
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