The 2.4 Å crystal structure of cholera toxin B subunit pentamer: Choleragenoid

Rong Guang Zhang, Mary L. Westbrook, Edwin M. Westbrook, David L. Scott, Zbyszek Otwinowski, Prakas R. Maulik, Robert A. Reed, G. Graham Shipley

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Cholera toxin, a heterohexameric AB5 enterotoxin released by Vibrio cholera, induces a profuse secretory diarrhea in susceptible hosts. Choleragenoid, the B subunit pentamer of cholera toxin, directs the enzymatic A subunit to its target by binding the GM1 gangliosides exposed on the luminal surface of intestinal epithelial cells. The crystal structure of choleragenoid has been independently solved and refined at 2.4 Å resolution by combining single isomorphous replacement with non-crystallographic symmetry averaging. The structure of the B subunits, and their pentameric arrangement, closely resembles that reported for the intact holotoxin, choleragen, the heat-labile enterotoxin from Escherichia coli, and for a choleragenoid-GM1 pentasaccharide complex. In the absence of the A subunit the central cavity of the B pentamer is a highly solvated channel. The binding of choleragenoid to the A subunit or to its receptor pentasaccharide modestly affects the local stereochemistry without perceptibly altering the subunit interface.

Original languageEnglish (US)
Pages (from-to)550-562
Number of pages13
JournalJournal of Molecular Biology
Volume251
Issue number4
StatePublished - 1995

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Cholera Toxin
Enterotoxins
G(M1) Ganglioside
Vibrio cholerae
Diarrhea
Hot Temperature
Epithelial Cells
Escherichia coli

Keywords

  • Cholera toxin
  • Choleragenoid
  • Crystal structure
  • Enterotoxins
  • Gangliosides

ASJC Scopus subject areas

  • Virology
  • Molecular Biology

Cite this

Zhang, R. G., Westbrook, M. L., Westbrook, E. M., Scott, D. L., Otwinowski, Z., Maulik, P. R., ... Graham Shipley, G. (1995). The 2.4 Å crystal structure of cholera toxin B subunit pentamer: Choleragenoid. Journal of Molecular Biology, 251(4), 550-562.

The 2.4 Å crystal structure of cholera toxin B subunit pentamer : Choleragenoid. / Zhang, Rong Guang; Westbrook, Mary L.; Westbrook, Edwin M.; Scott, David L.; Otwinowski, Zbyszek; Maulik, Prakas R.; Reed, Robert A.; Graham Shipley, G.

In: Journal of Molecular Biology, Vol. 251, No. 4, 1995, p. 550-562.

Research output: Contribution to journalArticle

Zhang, RG, Westbrook, ML, Westbrook, EM, Scott, DL, Otwinowski, Z, Maulik, PR, Reed, RA & Graham Shipley, G 1995, 'The 2.4 Å crystal structure of cholera toxin B subunit pentamer: Choleragenoid', Journal of Molecular Biology, vol. 251, no. 4, pp. 550-562.
Zhang RG, Westbrook ML, Westbrook EM, Scott DL, Otwinowski Z, Maulik PR et al. The 2.4 Å crystal structure of cholera toxin B subunit pentamer: Choleragenoid. Journal of Molecular Biology. 1995;251(4):550-562.
Zhang, Rong Guang ; Westbrook, Mary L. ; Westbrook, Edwin M. ; Scott, David L. ; Otwinowski, Zbyszek ; Maulik, Prakas R. ; Reed, Robert A. ; Graham Shipley, G. / The 2.4 Å crystal structure of cholera toxin B subunit pentamer : Choleragenoid. In: Journal of Molecular Biology. 1995 ; Vol. 251, No. 4. pp. 550-562.
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