TY - JOUR
T1 - The 3.8 Å resolution cryo-EM structure of Zika virus
AU - Sirohi, Devika
AU - Chen, Zhenguo
AU - Sun, Lei
AU - Klose, Thomas
AU - Pierson, Theodore C.
AU - Rossmann, Michael G.
AU - Kuhn, Richard J.
N1 - Funding Information:
We thank X. de Lamballerie (Emergence des Pathologies Virales, Aix-Marseille Université, Marseille, France) and the European Virus Archive Goes Global (EVAg) for consenting to the use of the H/PF/2013 ZIKV strain for this study under a material transfer agreement with EVAg's partner, Aix-Marseille Université, and we thank M. S. Diamond (Washington University, St. Louis, MO, USA) for sending the virus. We acknowledge E. Frye for help with sequence alignments. We also acknowledge the use of the Purdue cryo-EM facility. We are grateful to W. Jiang for his jspr program, which was used to perform the anisotropic magnification corrections, and Y. Liu for providing help in submitting the Protein Data Bank (PDB) coordinates. The work presented in this report was funded by the National Institute of Allergy and Infectious Diseases of the NIH through grants R01AI073755 and R01AI076331 to M.G.R. and R.J.K. T.C.P was supported by the intramural program of the National Institute of Allergy and Infectious Diseases. Supporting information for this research is provided in the supplementary materials. The atomic coordinates and cryo-EM density maps for the mature ZIKV are available at the PDB and Electron Microscopy Data Bank under accession codes 5IRE and EMD-8116, respectively.
PY - 2016/4/22
Y1 - 2016/4/22
N2 - The recent rapid spread of Zika virus and its unexpected linkage to birth defects and an autoimmune neurological syndrome have generated worldwide concern. Zika virus is a flavivirus like the dengue, yellow fever, and West Nile viruses.We present the 3.8 angstrom resolution structure of mature Zika virus, determined by cryo-electron microscopy (cryo-EM).The structure of Zika virus is similar to other known flavivirus structures, except for the ~10 amino acids that surround the Asn154 glycosylation site in each of the 180 envelope glycoproteins that make up the icosahedral shell. The carbohydrate moiety associated with this residue, which is recognizable in the cryo-EM electron density, may function as an attachment site of the virus to host cells. This region varies not only among Zika virus strains but also in other flaviviruses, which suggests that differences in this region may influence virus transmission and disease.
AB - The recent rapid spread of Zika virus and its unexpected linkage to birth defects and an autoimmune neurological syndrome have generated worldwide concern. Zika virus is a flavivirus like the dengue, yellow fever, and West Nile viruses.We present the 3.8 angstrom resolution structure of mature Zika virus, determined by cryo-electron microscopy (cryo-EM).The structure of Zika virus is similar to other known flavivirus structures, except for the ~10 amino acids that surround the Asn154 glycosylation site in each of the 180 envelope glycoproteins that make up the icosahedral shell. The carbohydrate moiety associated with this residue, which is recognizable in the cryo-EM electron density, may function as an attachment site of the virus to host cells. This region varies not only among Zika virus strains but also in other flaviviruses, which suggests that differences in this region may influence virus transmission and disease.
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U2 - 10.1126/science.aaf5316
DO - 10.1126/science.aaf5316
M3 - Article
C2 - 27033547
AN - SCOPUS:84962427374
SN - 0036-8075
VL - 352
SP - 467
EP - 470
JO - Science
JF - Science
IS - 6284
ER -