The ABC transporters in Candidatus Liberibacter asiaticus

Wenlin Li, Qian Cong, Jimin Pei, Lisa N. Kinch, Nick V. Grishin

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Candidatus Liberibacter asiaticus (Ca. L. asiaticus) is a Gram-negative bacterium and the pathogen of Citrus Greening disease (Huanglongbing, HLB). As a parasitic bacterium, Ca. L. asiaticus harbors ABC transporters that play important roles in exchanging chemical compounds between Ca. L. asiaticus and its host. Here, we analyzed all the ABC transporter-related proteins in Ca. L. asiaticus. We identified 14 ABC transporter systems and predicted their structures and substrate specificities. In-depth sequence and structure analysis including multiple sequence alignment, phylogenetic tree reconstruction, and structure comparison further support their function predictions. Our study shows that this bacterium could use these ABC transporters to import metabolites (amino acids and phosphates) and enzyme cofactors (choline, thiamine, iron, manganese, and zinc), resist to organic solvent, heavy metal, and lipid-like drugs, maintain the composition of the outer membrane (OM), and secrete virulence factors. Although the features of most ABC systems could be deduced from the abundant experimental data on their orthologs, we reported several novel observations within ABC system proteins. Moreover, we identified seven nontransport ABC systems that are likely involved in virulence gene expression regulation, transposon excision regulation, and DNA repair. Our analysis reveals several candidates for further studies to understand and control the disease, including the type I virulence factor secretion system and its substrate that are likely related to Ca. L. asiaticus pathogenicity and the ABC transporter systems responsible for bacterial OM biosynthesis that are good drug targets.

Original languageEnglish (US)
Pages (from-to)2614-2628
Number of pages15
JournalProteins: Structure, Function and Bioinformatics
Volume80
Issue number11
DOIs
StatePublished - Nov 2012

Fingerprint

ATP-Binding Cassette Transporters
Bacteria
Virulence Factors
DNA Repair
Virulence
Gene expression regulation
Membranes
Chemical compounds
Citrus
Sequence Alignment
Thiamine
Biosynthesis
Coenzymes
Gene Expression Regulation
Pathogens
Substrates
Manganese
Metabolites
Ports and harbors
Substrate Specificity

Keywords

  • ATPase
  • Function prediction
  • Genomic annotation
  • Multiple sequence alignment
  • Phylogenetic tree
  • Protein homology
  • Structure comparison
  • Transmembrane protein

ASJC Scopus subject areas

  • Biochemistry
  • Structural Biology
  • Molecular Biology

Cite this

The ABC transporters in Candidatus Liberibacter asiaticus. / Li, Wenlin; Cong, Qian; Pei, Jimin; Kinch, Lisa N.; Grishin, Nick V.

In: Proteins: Structure, Function and Bioinformatics, Vol. 80, No. 11, 11.2012, p. 2614-2628.

Research output: Contribution to journalArticle

Li, Wenlin ; Cong, Qian ; Pei, Jimin ; Kinch, Lisa N. ; Grishin, Nick V. / The ABC transporters in Candidatus Liberibacter asiaticus. In: Proteins: Structure, Function and Bioinformatics. 2012 ; Vol. 80, No. 11. pp. 2614-2628.
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