The Acellular Fraction of Stored Platelets Promotes Tumor Cell Invasion

Sean P. Dineen, Christina L. Roland, Jason E. Toombs, Marguerite Kelher, Christopher C. Silliman, Rolf A. Brekken, Carlton C. Barnett

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Background: Transfusion of blood products is a potentially life-saving treatment to correct deficits of volume status or oxygen delivery. Increasingly it has been recognized that transfusions also transmit immunosuppressive factors including cytokines and lipid mediators. Platelets are ubiquitously present in blood transfusions and contain numerous growth factors that may contribute to tumor growth. We hypothesized that such growth factors released during routine platelet storage promote cancer invasion. Materials and methods: Modified Boyden chamber transwell invasion assays were performed to determine if factors released into the plasma portion of stored platelets could induce tumor cell invasion. Results: Soluble mediators from stored platelets induce invasion in two pancreatic cancer cell lines (MIA PaCA-2, Pan02) and one breast cancer cell line (MDA-MB-231). Additionally, we show that vascular endothelial growth factor is present in the acellular fraction of stored platelets and that inhibition of vascular endothelial growth factor with bevacizumab reduces tumor cell invasion in vitro. Finally, we found that in vivo administration of this acellular fraction increases tumor angiogenesis. Conclusions: Components in stored platelets can promote the invasion of multiple cancer cell lines in vitro. These results indicate that factors in platelets may mediate deleterious effects associated with transfusion in cancer patients.

Original languageEnglish (US)
Pages (from-to)132-137
Number of pages6
JournalJournal of Surgical Research
Volume153
Issue number1
DOIs
StatePublished - May 1 2009

Keywords

  • VEGF
  • breast cancer
  • pancreatic cancer
  • platelet

ASJC Scopus subject areas

  • Surgery

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    Dineen, S. P., Roland, C. L., Toombs, J. E., Kelher, M., Silliman, C. C., Brekken, R. A., & Barnett, C. C. (2009). The Acellular Fraction of Stored Platelets Promotes Tumor Cell Invasion. Journal of Surgical Research, 153(1), 132-137. https://doi.org/10.1016/j.jss.2008.04.013