Abstract
The Drosophila vestigial gene is required for proliferation and differentiation of the adult wing and for differentiation of larval and adult muscle identity. Vestigial is part of a multi-protein transcription factor complex, which includes Scalloped, a TEAD-class DNA binding protein. Binding Scalloped is necessary for translocation of Vestigial into the nucleus. We show that Vestigial is extensively post-translationally modified and at least one of these modifications is required for proper function during development. We have shown that there is p38-dependent phosphorylation of Serine 215 in the carboxyl-terminal region of Vestigial. Phosphorylation of Serine 215 occurs in the nucleus and requires the presence of Scalloped. Comparison of a phosphomimetic and non-phosphorylatable mutant forms of Vestigial shows differences in the ability to rescue the wing and muscle phenotypes associated with a null vestigial allele.
Original language | English (US) |
---|---|
Pages (from-to) | 58-69 |
Number of pages | 12 |
Journal | Developmental Biology |
Volume | 425 |
Issue number | 1 |
DOIs | |
State | Published - May 1 2017 |
Externally published | Yes |
Keywords
- Drosophila
- P38
- Scalloped
- TEAD proteins
- Vestigial
- Vestigial-like
ASJC Scopus subject areas
- Molecular Biology
- Developmental Biology
- Cell Biology