The adipocyte as an important target cell for Trypanosoma cruzi infection

Terry P. Combs, Nagajyothi, Shankar Mukherjee, Cecilia J G De Almeida, Linda A. Jelicks, William Schubert, Ying Lin, David S. Jayabalan, Dazhi Zhao, Vicki L. Braunstein, Shira Landskroner-Eiger, Aisha Cordero, Stephen M. Factor, Louis M. Weiss, Michael P. Lisanti, Herbert B. Tanowitz, Philipp E. Scherer

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

Adipose tissue plays an active role in normal metabolic homeostasis as well as in the development of human disease. Beyond its obvious role as a depot for triglycerides, adipose tissue controls energy expenditure through secretion of several factors. Little attention has been given to the role of adipocytes in the pathogenesis of Chagas disease and the associated metabolic alterations. Our previous studies have indicated that hyperglycemia significantly increases parasitemia and mortality in mice infected with Trypanosoma cruzi. We determined the consequences of adipocyte infection in vitro and in vivo. Cultured 3T3-L1 adipocytes can be infected with high efficiency. Electron micrographs of infected cells revealed a large number of intracellular parasites that cluster around lipid droplets. Furthermore, infected adipocytes exhibited changes in expression levels of a number of different adipocyte-specific or adipocyte-enriched proteins. The adipocyte is therefore an important target cell during acute Chagas disease. Infection of adipocytes by T. cruzi profoundly influences the pattern of adipokines. During chronic infection, adipocytes may represent an important long-term reservoir for parasites from which relapse of infection can occur. We have demonstrated that acute infection has a unique metabolic profile with a high degree of local inflammation in adipose tissue, hypoadiponectinemia, hypoglycemia, and hypoinsulinemia but with relatively normal glucose disposal during an oral glucose tolerance test.

Original languageEnglish (US)
Pages (from-to)24085-24094
Number of pages10
JournalJournal of Biological Chemistry
Volume280
Issue number25
DOIs
StatePublished - Jun 24 2005

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Trypanosoma cruzi
Adipocytes
Tissue
Infection
Glucose
Adipokines
Adipose Tissue
Triglycerides
Chagas Disease
Lipids
Electrons
Parasites
Proteins
Metabolome
Parasitemia
Human Development
Acute Disease
Glucose Tolerance Test
Hypoglycemia
Hyperglycemia

ASJC Scopus subject areas

  • Biochemistry

Cite this

Combs, T. P., Nagajyothi, Mukherjee, S., De Almeida, C. J. G., Jelicks, L. A., Schubert, W., ... Scherer, P. E. (2005). The adipocyte as an important target cell for Trypanosoma cruzi infection. Journal of Biological Chemistry, 280(25), 24085-24094. https://doi.org/10.1074/jbc.M412802200

The adipocyte as an important target cell for Trypanosoma cruzi infection. / Combs, Terry P.; Nagajyothi; Mukherjee, Shankar; De Almeida, Cecilia J G; Jelicks, Linda A.; Schubert, William; Lin, Ying; Jayabalan, David S.; Zhao, Dazhi; Braunstein, Vicki L.; Landskroner-Eiger, Shira; Cordero, Aisha; Factor, Stephen M.; Weiss, Louis M.; Lisanti, Michael P.; Tanowitz, Herbert B.; Scherer, Philipp E.

In: Journal of Biological Chemistry, Vol. 280, No. 25, 24.06.2005, p. 24085-24094.

Research output: Contribution to journalArticle

Combs, TP, Nagajyothi, Mukherjee, S, De Almeida, CJG, Jelicks, LA, Schubert, W, Lin, Y, Jayabalan, DS, Zhao, D, Braunstein, VL, Landskroner-Eiger, S, Cordero, A, Factor, SM, Weiss, LM, Lisanti, MP, Tanowitz, HB & Scherer, PE 2005, 'The adipocyte as an important target cell for Trypanosoma cruzi infection', Journal of Biological Chemistry, vol. 280, no. 25, pp. 24085-24094. https://doi.org/10.1074/jbc.M412802200
Combs TP, Nagajyothi, Mukherjee S, De Almeida CJG, Jelicks LA, Schubert W et al. The adipocyte as an important target cell for Trypanosoma cruzi infection. Journal of Biological Chemistry. 2005 Jun 24;280(25):24085-24094. https://doi.org/10.1074/jbc.M412802200
Combs, Terry P. ; Nagajyothi ; Mukherjee, Shankar ; De Almeida, Cecilia J G ; Jelicks, Linda A. ; Schubert, William ; Lin, Ying ; Jayabalan, David S. ; Zhao, Dazhi ; Braunstein, Vicki L. ; Landskroner-Eiger, Shira ; Cordero, Aisha ; Factor, Stephen M. ; Weiss, Louis M. ; Lisanti, Michael P. ; Tanowitz, Herbert B. ; Scherer, Philipp E. / The adipocyte as an important target cell for Trypanosoma cruzi infection. In: Journal of Biological Chemistry. 2005 ; Vol. 280, No. 25. pp. 24085-24094.
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