The adipokine SAA3 is induced by interleukin-1β in mouse adipocytes

Grit Sommer; Sebastian Weise; Susan Kralisch; Philipp E. Scherer; Ulrike Lössner; Matthias Blüher; Michael Stumvoll; Mathias Fasshauer

doi:10.1002/jcb.21782

The adipokine SAA3 is induced by interleukin-1β in mouse adipocytes

Grit Sommer, Sebastian Weise, Susan Kralisch, Philipp E. Scherer, Ulrike Lössner, Matthias Blüher, Michael Stumvoll, Mathias Fasshauer

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Serum amyloid A (SAA) 3 has been characterized as an inflammatory adipocyte-secreted acute-phase reactant. In the current study, regulation of SAA3 by the proinflammatory and insulin resistance-inducing cytokine interleukin (IL)-1β was determined in 3T3-L1 and brown adipocytes. Interestingly, SAA3 mRNA and protein synthesis were dramatically increased by IL-1β in a time-dependent fashion with maximal induction after 24 h. Furthermore, IL-1β significantly induced SAA3 mRNA expression dose-dependently with maximal 36.4-fold upregulation seen at 2 ng/ml effector. Moreover, IL-1β-induced SAA3 expression was mediated by nuclear factor-κB and janus kinase 2. Taken together, our data show a potent upregulation of SAA3 by IL-1β.

Original language	English (US)
Pages (from-to)	2241-2247
Number of pages	7
Journal	Journal of Cellular Biochemistry
Volume	104
Issue number	6
DOIs	https://doi.org/10.1002/jcb.21782
State	Published - Aug 15 2008

Keywords

3T3-L1 adipocyte
Adipokine
Interleukin-1β
Serum amyloid A3

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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10.1002/jcb.21782

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title = "The adipokine SAA3 is induced by interleukin-1β in mouse adipocytes",

abstract = "Serum amyloid A (SAA) 3 has been characterized as an inflammatory adipocyte-secreted acute-phase reactant. In the current study, regulation of SAA3 by the proinflammatory and insulin resistance-inducing cytokine interleukin (IL)-1β was determined in 3T3-L1 and brown adipocytes. Interestingly, SAA3 mRNA and protein synthesis were dramatically increased by IL-1β in a time-dependent fashion with maximal induction after 24 h. Furthermore, IL-1β significantly induced SAA3 mRNA expression dose-dependently with maximal 36.4-fold upregulation seen at 2 ng/ml effector. Moreover, IL-1β-induced SAA3 expression was mediated by nuclear factor-κB and janus kinase 2. Taken together, our data show a potent upregulation of SAA3 by IL-1β.",

keywords = "3T3-L1 adipocyte, Adipokine, Interleukin-1β, Serum amyloid A3",

author = "Grit Sommer and Sebastian Weise and Susan Kralisch and Scherer, {Philipp E.} and Ulrike L{\"o}ssner and Matthias Bl{\"u}her and Michael Stumvoll and Mathias Fasshauer",

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doi = "10.1002/jcb.21782",

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T1 - The adipokine SAA3 is induced by interleukin-1β in mouse adipocytes

AU - Sommer, Grit

AU - Weise, Sebastian

AU - Kralisch, Susan

AU - Scherer, Philipp E.

AU - Lössner, Ulrike

AU - Blüher, Matthias

AU - Stumvoll, Michael

AU - Fasshauer, Mathias

PY - 2008/8/15

Y1 - 2008/8/15

N2 - Serum amyloid A (SAA) 3 has been characterized as an inflammatory adipocyte-secreted acute-phase reactant. In the current study, regulation of SAA3 by the proinflammatory and insulin resistance-inducing cytokine interleukin (IL)-1β was determined in 3T3-L1 and brown adipocytes. Interestingly, SAA3 mRNA and protein synthesis were dramatically increased by IL-1β in a time-dependent fashion with maximal induction after 24 h. Furthermore, IL-1β significantly induced SAA3 mRNA expression dose-dependently with maximal 36.4-fold upregulation seen at 2 ng/ml effector. Moreover, IL-1β-induced SAA3 expression was mediated by nuclear factor-κB and janus kinase 2. Taken together, our data show a potent upregulation of SAA3 by IL-1β.

AB - Serum amyloid A (SAA) 3 has been characterized as an inflammatory adipocyte-secreted acute-phase reactant. In the current study, regulation of SAA3 by the proinflammatory and insulin resistance-inducing cytokine interleukin (IL)-1β was determined in 3T3-L1 and brown adipocytes. Interestingly, SAA3 mRNA and protein synthesis were dramatically increased by IL-1β in a time-dependent fashion with maximal induction after 24 h. Furthermore, IL-1β significantly induced SAA3 mRNA expression dose-dependently with maximal 36.4-fold upregulation seen at 2 ng/ml effector. Moreover, IL-1β-induced SAA3 expression was mediated by nuclear factor-κB and janus kinase 2. Taken together, our data show a potent upregulation of SAA3 by IL-1β.

KW - 3T3-L1 adipocyte

KW - Adipokine

KW - Interleukin-1β

KW - Serum amyloid A3

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The adipokine SAA3 is induced by interleukin-1β in mouse adipocytes

Abstract

Keywords

ASJC Scopus subject areas

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