The alloreactive T cell response against the class Ib molecule H2-M3 is specific for high affinity peptides

Vikram M. Dabhi, Rolf Hovik, Luc Van Kaer, Kirsten Fischer Lindahl

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

MHC class Ib molecule H2-M3 presents N-formylated peptides to CD8+ CTLs. Endogenous formylated peptides can come from the N-terminus of each of the 13 proteins encoded by the mitochondrial genome. In peptide competition assays, two of these peptides bind with high affinity, six bind with intermediate affinity, three bind with low affinity, and two do not bind measurably. Alloreactive CTLs from M3-specific, mixed lymphocyte cultures responded strongly against the two peptides with high affinity for M3, occasionally to peptides with intermediate affinity, and not at all to the rest. Long term lines and CTL clones reacted with only the high affinity peptides, demonstrating that alloreactive CTLs depend on specific peptides and that peptide affinity for class I correlates with alloantigenicity.

Original languageEnglish (US)
Pages (from-to)5171-5178
Number of pages8
JournalJournal of Immunology
Volume161
Issue number10
StatePublished - Nov 15 1998

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Dabhi, V. M., Hovik, R., Van Kaer, L., & Lindahl, K. F. (1998). The alloreactive T cell response against the class Ib molecule H2-M3 is specific for high affinity peptides. Journal of Immunology, 161(10), 5171-5178.