The anatomical basis for wrinkles

Joel E. Pessa, Hang Nguyen, George B. John, Philipp E. Scherer

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: Light and electron microscopy have not identified a distinct anatomical structure associated with either skin wrinkles or creases, and a histological difference between wrinkled and adjacent skin has not been identified. Objectives: The authors investigate whether facial wrinkles are related to underlying lymphatic vessels and perilymphatic fat. Methods: Lymphatic vessels with a specialized tube of perilymphatic fat were identified beneath palmar creases. Sections of skin, adipose tissue, and muscle were harvested from each of 13 cadavers. Three sites were investigated: the transverse forehead crease, lateral orbicularis oculi wrinkle (crows feet), and the nasojugal crease. The tissue was paraffin embedded and processed. Two-step indirect immunohistochemistry was performed, and images were examined using laser confocal microscopy. Measurements were taken with software. Results: Every wrinkle examined was found above and within 1 mm of a major lymphatic vessel and its surrounding tube of adipose tissue. The results satisfied our null hypothesis and were statistically significant. Lymphatic vessels were identified by positive immunofluorescence as well as histological criteria. These findings have been further validated by fluorochrome tracer studies. Conclusions: An anatomical basis for wrinkles was identified among the specimens studied. Lymphatic vessels, along with the surrounding distinct perilymphatic fat, traveled directly beneath wrinkles and creases. Lymphatic dysregulation leads to inflammation, scarring, and fibrosis, but inadvertent injection of these vessels can be avoided with anatomical knowledge.

Original languageEnglish (US)
Pages (from-to)227-234
Number of pages8
JournalAesthetic Surgery Journal
Volume34
Issue number2
DOIs
StatePublished - Feb 2014

Fingerprint

Lymphatic Vessels
Fats
Confocal Microscopy
Skin
Adipose Tissue
Crows
Forehead
Fluorescent Dyes
Cadaver
Paraffin
Cicatrix
Fluorescent Antibody Technique
Foot
Electron Microscopy
Fibrosis
Software
Immunohistochemistry
Inflammation
Light
Muscles

Keywords

  • facial surgery
  • fillers
  • lasers
  • liposculpting
  • resurfacing
  • skin redundancy
  • wrinkles

ASJC Scopus subject areas

  • Surgery

Cite this

The anatomical basis for wrinkles. / Pessa, Joel E.; Nguyen, Hang; John, George B.; Scherer, Philipp E.

In: Aesthetic Surgery Journal, Vol. 34, No. 2, 02.2014, p. 227-234.

Research output: Contribution to journalArticle

Pessa, Joel E. ; Nguyen, Hang ; John, George B. ; Scherer, Philipp E. / The anatomical basis for wrinkles. In: Aesthetic Surgery Journal. 2014 ; Vol. 34, No. 2. pp. 227-234.
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