The anti-inflammatory effects of heat shock protein 72 involve inhibition of high-mobility-group box 1 release and proinflammatory function in macrophages

Daolin Tang, Rui Kang, Weimin Xiao, Haichao Wang, Stuart K. Calderwood, Xianzhong Xiao

Research output: Contribution to journalArticle

93 Scopus citations

Abstract

High-mobility-group box 1 (HMGB1), a nuclear protein, has recently been identified as an important mediator of local and systemic inflammatory diseases when released into the extracellular milieu. Anti-inflammatory regulation by the stress response is an effective autoprotective mechanism when the host encounters harmful stimuli, but the mechanism of action remains incompletely delineated. In this study, we demonstrate that increases in levels of a major stress-inducible protein, heat shock protein 72 (Hsp72) by gene transfection attenuated LPS- or TNF-α-induced HMGB1 cytoplasmic translocation and release. The mechanisms involved inhibition of the chromosome region maintenance 1 (CRM1)-dependent nuclear export pathway. Overexpression of Hsp72 inhibited CRM1 translocation and interaction between HMGB1 and CRM1 in macrophages post-LPS and TNF-α treatment. In addition, overexpression of Hsp72 strongly inhibited HMGB1-induced cytokine (TNF-α, IL-1β) expression and release, which correlated closely with: 1) inhibition of the MAP kinases (p38, JNK, and ERK); and 2) inhibition of the NF-κB pathway. Taken together, these experiments suggest that the anti-inflammatory activity of Hsp72 is achieved by interfering with both the release and proinflammatory function of HMGB1. Our experimental data provide important insights into the anti-inflammatory mechanisms of heat shock protein protection.

Original languageEnglish (US)
Pages (from-to)1236-1244
Number of pages9
JournalJournal of Immunology
Volume179
Issue number2
DOIs
Publication statusPublished - Jul 15 2007
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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