The assembly of tubulin into membranes

Howard Feit, Jerry W. Shay

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Tubulin in high-speed supernatants of brain undergoes an alternate form of polymerization into structures that resemble membranes rather than microtubules. The reaction required elevated temperature (37°C) and was prevented by 1mM CaCl2 or 10-4 M maytansine. The membraneous material was composed of tubulin (80%) and microtubule-associated proteins (8%) and contained phospholipids. The tubulin was identified on the basis of comigration in two-dimensional gel electrophoresis and colchicine-binding activity.

Original languageEnglish (US)
Pages (from-to)324-331
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume94
Issue number1
DOIs
StatePublished - May 14 1980

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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