The ATPase core of a clathrin uncoating protein

T. G. Chappell, B. B. Konforti, S. L. Schmid, J. E. Rothman

Research output: Contribution to journalArticle

215 Citations (Scopus)

Abstract

Chymotryptic digestion of bovine brain uncoating ATPase produced a 60-kDa fragment that was subsequently proteolyzed to 44 kDa. Loss of clathrin cage uncoating activity paralleled the conversion of the intact 70-kDa enzyme to the 60-kDa fragment, while clathrin binding activity was lost as the 60-kDa fragment was degraded to 44 kDa. This 44-kDa fragment has been purified to homogeneity and characterized as a clathrin-independent ATPase. The 44-kDa ATPase domain has been localized within the intact enzyme by the use of amino-terminal specific antibodies. This localization relates to the conserved nature of the 70-kDa heat shock protein family, of which bovine brain uncoating ATPase is a constitutively expressed member.

Original languageEnglish (US)
Pages (from-to)746-751
Number of pages6
JournalJournal of Biological Chemistry
Volume262
Issue number2
StatePublished - 1987

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HSC70 Heat-Shock Proteins
Clathrin
Adenosine Triphosphatases
Brain
HSP70 Heat-Shock Proteins
Enzymes
Digestion
Antibodies

ASJC Scopus subject areas

  • Biochemistry

Cite this

Chappell, T. G., Konforti, B. B., Schmid, S. L., & Rothman, J. E. (1987). The ATPase core of a clathrin uncoating protein. Journal of Biological Chemistry, 262(2), 746-751.

The ATPase core of a clathrin uncoating protein. / Chappell, T. G.; Konforti, B. B.; Schmid, S. L.; Rothman, J. E.

In: Journal of Biological Chemistry, Vol. 262, No. 2, 1987, p. 746-751.

Research output: Contribution to journalArticle

Chappell, TG, Konforti, BB, Schmid, SL & Rothman, JE 1987, 'The ATPase core of a clathrin uncoating protein', Journal of Biological Chemistry, vol. 262, no. 2, pp. 746-751.
Chappell TG, Konforti BB, Schmid SL, Rothman JE. The ATPase core of a clathrin uncoating protein. Journal of Biological Chemistry. 1987;262(2):746-751.
Chappell, T. G. ; Konforti, B. B. ; Schmid, S. L. ; Rothman, J. E. / The ATPase core of a clathrin uncoating protein. In: Journal of Biological Chemistry. 1987 ; Vol. 262, No. 2. pp. 746-751.
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