The basement membrane of hair follicle stem cells is a muscle cell niche

Hironobu Fujiwara, Manuela Ferreira, Giacomo Donati, Denise K. Marciano, James M. Linton, Yuya Sato, Andrea Hartner, Kiyotoshi Sekiguchi, Louis F. Reichardt, Fiona M. Watt

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162 Scopus citations

Abstract

The hair follicle bulge in the epidermis associates with the arrector pili muscle (APM) that is responsible for piloerection ("goosebumps"). We show that stem cells in the bulge deposit nephronectin into the underlying basement membrane, thus regulating the adhesion of mesenchymal cells expressing the nephronectin receptor, α8β1 integrin, to the bulge. Nephronectin induces α8 integrin-positive mesenchymal cells to upregulate smooth muscle markers. In nephronectin knockout mice, fewer arrector pili muscles form in the skin, and they attach to the follicle above the bulge, where there is compensatory upregulation of the nephronectin family member EGFL6. Deletion of α8 integrin also abolishes selective APM anchorage to the bulge. Nephronectin is a Wnt target; epidermal β-catenin activation upregulates epidermal nephronectin and dermal α8 integrin expression. Thus, bulge stem cells, via nephronectin expression, create a smooth muscle cell niche and act as tendon cells for the APM. Our results reveal a functional role for basement membrane heterogeneity in tissue patterning.

Original languageEnglish (US)
Pages (from-to)577-589
Number of pages13
JournalCell
Volume144
Issue number4
DOIs
StatePublished - Feb 18 2011

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Fujiwara, H., Ferreira, M., Donati, G., Marciano, D. K., Linton, J. M., Sato, Y., Hartner, A., Sekiguchi, K., Reichardt, L. F., & Watt, F. M. (2011). The basement membrane of hair follicle stem cells is a muscle cell niche. Cell, 144(4), 577-589. https://doi.org/10.1016/j.cell.2011.01.014