The BDGP gene disruption project: Single transposon insertions associated with 40% of Drosophila genes

Hugo J. Bellen, Robert W. Levis, Guochun Liao, Yuchun He, Joseph W. Carlson, Garson Tsang, Martha Evans-Holm, P. Robin Hiesinger, Karen L. Schulze, Gerald M. Rubin, Roger A. Hoskins, Allan C. Spradling

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Abstract

The Berkeley Drosophila Genome Project (BDGP) strives to disrupt each Drosophila gene by the insertion of a single transposable element. As part of this effort, transposons in >30,000 fly strains were localized and analyzed relative to predicted Drosophila gene structures. Approximately 6300 lines that maximize genomic coverage were selected to be sent to the Bloomington Stock Center for public distribution, bringing the size of the BDGP gene disruption collection to 7140 lines. It now includes individual lines predicted to disrupt 5362 of the 13,666 currently annotated Drosophila genes (39%). Other lines contain an insertion at least 2 kb from others in the collection and likely mutate additional incompletely annotated or uncharacterized genes and chromosomal regulatory elements. The remaining strains contain insertions likely to disrupt alternative gene promoters or to allow gene misexpression. The expanded BDGP gene disruption collection provides a public resource that will facilitate the application of Drosophila genetics to diverse biological problems. Finally, the project reveals new insight into how transposons interact with a eukaryotic genome and helps define optimal strategies for using insertional mutagenesis as a genomic tool.

Original languageEnglish (US)
Pages (from-to)761-781
Number of pages21
JournalGenetics
Volume167
Issue number2
DOIs
StatePublished - Jun 2004

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Drosophila
Genome
Genes
Insertional Mutagenesis
DNA Transposable Elements
Regulator Genes
Diptera

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Bellen, H. J., Levis, R. W., Liao, G., He, Y., Carlson, J. W., Tsang, G., ... Spradling, A. C. (2004). The BDGP gene disruption project: Single transposon insertions associated with 40% of Drosophila genes. Genetics, 167(2), 761-781. https://doi.org/10.1534/genetics.104.026427

The BDGP gene disruption project : Single transposon insertions associated with 40% of Drosophila genes. / Bellen, Hugo J.; Levis, Robert W.; Liao, Guochun; He, Yuchun; Carlson, Joseph W.; Tsang, Garson; Evans-Holm, Martha; Hiesinger, P. Robin; Schulze, Karen L.; Rubin, Gerald M.; Hoskins, Roger A.; Spradling, Allan C.

In: Genetics, Vol. 167, No. 2, 06.2004, p. 761-781.

Research output: Contribution to journalArticle

Bellen, HJ, Levis, RW, Liao, G, He, Y, Carlson, JW, Tsang, G, Evans-Holm, M, Hiesinger, PR, Schulze, KL, Rubin, GM, Hoskins, RA & Spradling, AC 2004, 'The BDGP gene disruption project: Single transposon insertions associated with 40% of Drosophila genes', Genetics, vol. 167, no. 2, pp. 761-781. https://doi.org/10.1534/genetics.104.026427
Bellen, Hugo J. ; Levis, Robert W. ; Liao, Guochun ; He, Yuchun ; Carlson, Joseph W. ; Tsang, Garson ; Evans-Holm, Martha ; Hiesinger, P. Robin ; Schulze, Karen L. ; Rubin, Gerald M. ; Hoskins, Roger A. ; Spradling, Allan C. / The BDGP gene disruption project : Single transposon insertions associated with 40% of Drosophila genes. In: Genetics. 2004 ; Vol. 167, No. 2. pp. 761-781.
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