The Burkholderia mallei BmaR3-BmaI3 quorum-sensing system produces and responds to N-3-hydroxy-octanoyl homoserine lactone

Breck A. Duerkop, Jake P. Herman, Ricky L. Ulrich, Mair E A Churchill, E. Peter Greenberg

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Burkholderia mallei has two acyl-homoserine lactone (acyl-HSL) signal generator-receptor pairs and two additional signal receptors, all of which contribute to virulence. We show that B. mallei produces N-3-hydroxyoctanoyl HSL (3OHC8-HSL) but a bmaI3 mutant does not. Recombinant Escherichia coli expressing BmaI3 produces hydroxylated acyl-HSLs, with 3OHC8-HSL being the most abundant compound. In recombinant E. coli, BmaR3 responds to 3OHC8-HSL but not to other acyl-HSLs. These data indicate that the signal for BmaR3-BmaI3 quorum sensing is 3OHC8-HSL.

Original languageEnglish (US)
Pages (from-to)5137-5141
Number of pages5
JournalJournal of bacteriology
Volume190
Issue number14
DOIs
StatePublished - Jul 2008

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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