The Burkholderia mallei BmaR3-BmaI3 quorum-sensing system produces and responds to N-3-hydroxy-octanoyl homoserine lactone

Breck A. Duerkop, Jake P. Herman, Ricky L. Ulrich, Mair E A Churchill, E. Peter Greenberg

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Burkholderia mallei has two acyl-homoserine lactone (acyl-HSL) signal generator-receptor pairs and two additional signal receptors, all of which contribute to virulence. We show that B. mallei produces N-3-hydroxyoctanoyl HSL (3OHC8-HSL) but a bmaI3 mutant does not. Recombinant Escherichia coli expressing BmaI3 produces hydroxylated acyl-HSLs, with 3OHC8-HSL being the most abundant compound. In recombinant E. coli, BmaR3 responds to 3OHC8-HSL but not to other acyl-HSLs. These data indicate that the signal for BmaR3-BmaI3 quorum sensing is 3OHC8-HSL.

Original languageEnglish (US)
Pages (from-to)5137-5141
Number of pages5
JournalJournal of Bacteriology
Volume190
Issue number14
DOIs
StatePublished - Jul 2008

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Burkholderia mallei
Quorum Sensing
Acyl-Butyrolactones
Escherichia coli
Virulence
homoserine lactone

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

The Burkholderia mallei BmaR3-BmaI3 quorum-sensing system produces and responds to N-3-hydroxy-octanoyl homoserine lactone. / Duerkop, Breck A.; Herman, Jake P.; Ulrich, Ricky L.; Churchill, Mair E A; Greenberg, E. Peter.

In: Journal of Bacteriology, Vol. 190, No. 14, 07.2008, p. 5137-5141.

Research output: Contribution to journalArticle

Duerkop, Breck A. ; Herman, Jake P. ; Ulrich, Ricky L. ; Churchill, Mair E A ; Greenberg, E. Peter. / The Burkholderia mallei BmaR3-BmaI3 quorum-sensing system produces and responds to N-3-hydroxy-octanoyl homoserine lactone. In: Journal of Bacteriology. 2008 ; Vol. 190, No. 14. pp. 5137-5141.
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