The C-terminal domain of the largest subunit of RNA polymerase II interacts with a novel set of serine/arginine-rich proteins

Anton Yuryev, Meera Patturajan, Ying Litingtung, Ravi V. Joshi, Cristl Gentile, Maha Gebara, Jeffry L. Corden

Research output: Contribution to journalArticle

272 Citations (Scopus)

Abstract

Although transcription and pre-mRNA processing are colocalized in eukaryotic nuclei, molecules linking these processes have not previously been described. We have identified four novel rat proteins by their ability to interact with the repetitive C-terminal domain (CTD) of RNA polymerase II in a yeast two-hybrid assay. A yeast homolog of one of the rat proteins has also been shown to interact with the CTD. These CTD-binding proteins are all similar to the SR (serine/arginine-rich) family of proteins that have been shown to be involved in constitutive and regulated splicing. In addition to alternating Ser-Arg domains, these proteins each contain discrete N-terminal or C-terminal CTD-binding domains. We have identified SR-related proteins in a complex that can be immunoprecipitated from nuclear extracts with antibodies directed against RNA polymerase II. In addition, in vitro splicing is inhibited either by an antibody directed against the CTD or by wild-type but not mutant CTD peptides. Thus, these results suggest that the CTD and a set of CTD-binding proteins may act to physically and functionally link transcription and pre-mRNA processing.

Original languageEnglish (US)
Pages (from-to)6975-6980
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number14
DOIs
StatePublished - Jul 9 1996

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Serine
Arginine
RNA Polymerase II
RNA Precursors
Proteins
Two-Hybrid System Techniques
Antibodies
Yeasts
RNA polymerase II largest subunit
Protein Domains
C-terminal binding protein

Keywords

  • arginine-rich proteins
  • RNA processing
  • serine
  • transcription
  • two-hybrid assay

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

The C-terminal domain of the largest subunit of RNA polymerase II interacts with a novel set of serine/arginine-rich proteins. / Yuryev, Anton; Patturajan, Meera; Litingtung, Ying; Joshi, Ravi V.; Gentile, Cristl; Gebara, Maha; Corden, Jeffry L.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, No. 14, 09.07.1996, p. 6975-6980.

Research output: Contribution to journalArticle

Yuryev, Anton ; Patturajan, Meera ; Litingtung, Ying ; Joshi, Ravi V. ; Gentile, Cristl ; Gebara, Maha ; Corden, Jeffry L. / The C-terminal domain of the largest subunit of RNA polymerase II interacts with a novel set of serine/arginine-rich proteins. In: Proceedings of the National Academy of Sciences of the United States of America. 1996 ; Vol. 93, No. 14. pp. 6975-6980.
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