The Caenorhabditis elegans septin complex is nonpolar

Corinne M. John, Richard K. Hite, Christine S. Weirich, Daniel J. Fitzgerald, Hatim Jawhari, Mahamadou Faty, Dominik Schläpfer, Ruth Kroschewski, Fritz K. Winkler, Tom Walz, Yves Barral, Michel O. Steinmetz

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Septins are conserved GTPases that form heteromultimeric complexes and assemble into filaments that play a critical role in cell division and polarity. Results from budding and fission yeast indicate that septin complexes form around a tetrameric core. However, the molecular structure of the core and its influence on the polarity of septin complexes and filaments is poorly defined. The septin complex of the nematode Caenorhabditis elegans is formed entirely by the core septins UNC-59 and UNC-61. We show that UNC-59 and UNC-61 form a dimer of coiled-coil-mediated heterodimers. By electron microscopy, this heterotetramer appears as a linear arrangement of four densities representing the four septin subunits. Fusion of GFP to the N termini of UNC-59 and UNC-61 and subsequent electron microscopic visualization suggests that the sequence of septin subunits is UNC-59/UNC-61/UNC-61/UNC-59. Visualization of GFP extensions fused to the extremity of the C-terminal coiled coils indicates that these extend laterally from the heterotetrameric core. Together, our study establishes that the septin core complex is symmetric, and suggests that septins form nonpolar filaments.

Original languageEnglish (US)
Pages (from-to)3296-3307
Number of pages12
JournalEMBO Journal
Volume26
Issue number14
DOIs
StatePublished - Jul 25 2007

Fingerprint

Septins
Caenorhabditis elegans
Visualization
Cell Polarity
Saccharomycetales
Schizosaccharomyces
GTP Phosphohydrolases
Molecular Structure
Cell Division
Dimers
Yeast
Molecular structure
Electron microscopy
Electron Microscopy
Fusion reactions
Extremities
Cells
Electrons

Keywords

  • Cell polarity
  • Coiled coil
  • Cytoskeleton
  • Electron microscopy
  • Septin

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

John, C. M., Hite, R. K., Weirich, C. S., Fitzgerald, D. J., Jawhari, H., Faty, M., ... Steinmetz, M. O. (2007). The Caenorhabditis elegans septin complex is nonpolar. EMBO Journal, 26(14), 3296-3307. https://doi.org/10.1038/sj.emboj.7601775

The Caenorhabditis elegans septin complex is nonpolar. / John, Corinne M.; Hite, Richard K.; Weirich, Christine S.; Fitzgerald, Daniel J.; Jawhari, Hatim; Faty, Mahamadou; Schläpfer, Dominik; Kroschewski, Ruth; Winkler, Fritz K.; Walz, Tom; Barral, Yves; Steinmetz, Michel O.

In: EMBO Journal, Vol. 26, No. 14, 25.07.2007, p. 3296-3307.

Research output: Contribution to journalArticle

John, CM, Hite, RK, Weirich, CS, Fitzgerald, DJ, Jawhari, H, Faty, M, Schläpfer, D, Kroschewski, R, Winkler, FK, Walz, T, Barral, Y & Steinmetz, MO 2007, 'The Caenorhabditis elegans septin complex is nonpolar', EMBO Journal, vol. 26, no. 14, pp. 3296-3307. https://doi.org/10.1038/sj.emboj.7601775
John CM, Hite RK, Weirich CS, Fitzgerald DJ, Jawhari H, Faty M et al. The Caenorhabditis elegans septin complex is nonpolar. EMBO Journal. 2007 Jul 25;26(14):3296-3307. https://doi.org/10.1038/sj.emboj.7601775
John, Corinne M. ; Hite, Richard K. ; Weirich, Christine S. ; Fitzgerald, Daniel J. ; Jawhari, Hatim ; Faty, Mahamadou ; Schläpfer, Dominik ; Kroschewski, Ruth ; Winkler, Fritz K. ; Walz, Tom ; Barral, Yves ; Steinmetz, Michel O. / The Caenorhabditis elegans septin complex is nonpolar. In: EMBO Journal. 2007 ; Vol. 26, No. 14. pp. 3296-3307.
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