The cancer-testis antigens SPANX-A/C/D and CTAG2 promote breast cancer invasion

Erin A. Maine, Jill M. Westcott, Amanda M. Prechtl, Tuyen T. Dang, Angelique W. Whitehurst, Gray W. Pearson

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Genes that are normally biased towards expression in the testis are often induced in tumor cells. These gametogenic genes, known as cancer-testis antigens (CTAs), have been extenstively investigated as targets for immunotherapy. However, despite their frequent detection, the degree to which CTAs support neoplastic invasion is poorly understood. Here, we find that the CTA genes SPANX-A/C/D and CTAG2 are coordinately induced in breast cancer cells and regulate distinct features of invasive behavior. Our functional analysis revealed that CTAG2 interacts with Pericentrin at the centrosome and is necessary for directional migration. Conversely, SPANX-A/C/D interacts with Lamin A/C at the inner nuclear membrane and is required for the formation of actin-rich cellular protrusions that reorganize the extracellular matrix. Importantly, SPANX-A/C/D was required for breast cancer cells to spontaneously metastasize to the lung, demonstrating that CTA reactivation can be critical for invasion dependent phenotypes in vivo. Moreover, elevated SPANX-A/C/D expression in breast cancer patient tumors correlated with poor outcome. Together, our results suggest that distinct CTAs promote tumor progression by regulating complementary cellular functions that are integrated together to induce invasive behavior.

Original languageEnglish (US)
Pages (from-to)14708-14726
Number of pages19
JournalOncotarget
Volume7
Issue number12
DOIs
StatePublished - Mar 22 2016

Fingerprint

Testicular Neoplasms
Breast Neoplasms
Antigens
Cell Surface Extensions
Lamin Type A
Genes
Centrosome
Nuclear Envelope
Neoplasm Antigens
Immunotherapy
Extracellular Matrix
Testis
Actins
Lung Neoplasms
Neoplasms
Phenotype

Keywords

  • Breast cancer
  • Cancer-testis antigen
  • Extracellular matrix
  • Invasion
  • Metastasis

ASJC Scopus subject areas

  • Oncology

Cite this

The cancer-testis antigens SPANX-A/C/D and CTAG2 promote breast cancer invasion. / Maine, Erin A.; Westcott, Jill M.; Prechtl, Amanda M.; Dang, Tuyen T.; Whitehurst, Angelique W.; Pearson, Gray W.

In: Oncotarget, Vol. 7, No. 12, 22.03.2016, p. 14708-14726.

Research output: Contribution to journalArticle

Maine, Erin A. ; Westcott, Jill M. ; Prechtl, Amanda M. ; Dang, Tuyen T. ; Whitehurst, Angelique W. ; Pearson, Gray W. / The cancer-testis antigens SPANX-A/C/D and CTAG2 promote breast cancer invasion. In: Oncotarget. 2016 ; Vol. 7, No. 12. pp. 14708-14726.
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