The canine spleen in oxygen transport: Gas exchange and hemodynamic responses to splenectomy

Connie C W Hsia, Robert L. Johnson, D. Merrill Dane, Eugene Y. Wu, Aaron S. Estrera, Harrieth E. Wagner, Peter D. Wagner

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In athletic animals the spleen induces acute polycythemia by dynamic contraction that releases red blood cells into the circulation in response to increased O2 demand and metabolic stress; when energy demand is relieved, the polycythemia is rapidly reversed by splenic relaxation. We have shown in adult foxhounds that splenectomy eliminates exerciseinduced polycythemia, thereby reducing peak O2 uptake and lung diffusing capacity for carbon monoxide (DLCO) as well as exaggerating preexisting DLCO impairment imposed by pneumonectomy (Dane DM, Hsia CC, Wu EY, Hogg RT, Hogg DC, Estrera AS, Johnson RL Jr. J Appl Physiol 101: 289-297, 2006). To examine whether the postsplenectomy reduction in DLCO leads to abnormalities in O2 diffusion, ventilation-perfusion inequality, or hemodynamic function, we studied these animals via the multiple inert gas elimination technique at rest and during exercise at a constant workload equivalent to 50% or 80% of peak O2 uptake while breathing 21% and 14% O2 in balanced order. From rest to exercise after splenectomy, minute ventilation was significantly elevated with respect to O2 uptake compared with exercise before splenectomy; cardiac output, O2 delivery, and mean pulmonary and systemic arterial blood pressures were 10-20% lower, while O2 extraction was elevated with respect to O2 uptake. Ventilation-perfusion inequality was unchanged, but O2 diffusing capacities of lung (DLO2) and peripheral tissue during exercise were lower with respect to cardiac output postsplenectomy by 32% and 25%, respectively. The relationship between DLO2 and DLCO was unchanged by splenectomy. We conclude that the canine spleen regulates both convective and diffusive O2 transport during exercise to increase maximal O2 uptake.

Original languageEnglish (US)
Pages (from-to)1496-1505
Number of pages10
JournalJournal of Applied Physiology
Volume103
Issue number5
DOIs
StatePublished - Nov 2007

Fingerprint

Splenectomy
Polycythemia
Canidae
Spleen
Gases
Hemodynamics
Oxygen
Lung Volume Measurements
Ventilation
Cardiac Output
Perfusion
Noble Gases
Physiological Stress
Pneumonectomy
Carbon Monoxide
Workload
Sports
Arterial Pressure
Respiration
Erythrocytes

Keywords

  • Alveolararterial oxygen tension gradient
  • Convective oxygen delivery
  • Diffusing capacity for oxygen
  • Dog
  • Hemodynamic function
  • Pneumonectomy
  • Ventilation-perfusion distributions

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

The canine spleen in oxygen transport : Gas exchange and hemodynamic responses to splenectomy. / Hsia, Connie C W; Johnson, Robert L.; Dane, D. Merrill; Wu, Eugene Y.; Estrera, Aaron S.; Wagner, Harrieth E.; Wagner, Peter D.

In: Journal of Applied Physiology, Vol. 103, No. 5, 11.2007, p. 1496-1505.

Research output: Contribution to journalArticle

Hsia, Connie C W ; Johnson, Robert L. ; Dane, D. Merrill ; Wu, Eugene Y. ; Estrera, Aaron S. ; Wagner, Harrieth E. ; Wagner, Peter D. / The canine spleen in oxygen transport : Gas exchange and hemodynamic responses to splenectomy. In: Journal of Applied Physiology. 2007 ; Vol. 103, No. 5. pp. 1496-1505.
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