The capsule-encoding viaB locus reduces intestinal inflammation by a Salmonella pathogenicity island 1-independent mechanism

Takeshi Haneda, Sebastian E. Winter, Brian P. Butler, R. Paul Wilson, Çagla Tükel, Maria G. Winter, Ivan Godinez, Renée M. Tsolis, Andreas J. Bäumler

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Salmonella enterica serotype Typhimurium elicits acute neutrophil influx in the human intestinal mucosa within 1 or 2 days after infection, resulting in inflammatory diarrhea. In contrast, no overt symptoms are observed within the first 1 or 2 weeks after infection with S. enterica serotype Typhi. Here we show that introduction of the capsule-encoding viaB locus of serotype Typhi reduced the ability of serotype Typhimurium to elicit acute intestinal inflammation in a streptomycin-pretreated mouse model. Serotype Typhimurium requires a functional invasion-associated type III secretion system (type III secretion system 1 [T3SS-1]) to elicit cecal inflammation within 48 h after infection of streptomycin-pretreated mice, and the presence of the viaB locus reduced its invasiveness for human intestinal epithelial cells in vitro. However, a reduced activity of T3SS-1 could not account for the ability of the viaB locus to attenuate cecal inflammation, because introduction of the viaB locus into an invasion-deficient serotype Typhimurium strain (invA mutant) resulted in a significant reduction of pathology and inflammatory cytokine expression in the cecum 5 days after infection of mice. We conclude that a T3SS-1-independent mechanism contributes to the ability of the viaB locus to reduce intestinal inflammation.

Original languageEnglish (US)
Pages (from-to)2932-2942
Number of pages11
JournalInfection and Immunity
Volume77
Issue number7
DOIs
StatePublished - Jul 2009

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Genomic Islands
Salmonella
Capsules
Inflammation
Salmonella enterica
Streptomycin
Infection
Cecum
Intestinal Mucosa
Serogroup
Diarrhea
Neutrophils
Epithelial Cells
Pathology
Cytokines

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

The capsule-encoding viaB locus reduces intestinal inflammation by a Salmonella pathogenicity island 1-independent mechanism. / Haneda, Takeshi; Winter, Sebastian E.; Butler, Brian P.; Wilson, R. Paul; Tükel, Çagla; Winter, Maria G.; Godinez, Ivan; Tsolis, Renée M.; Bäumler, Andreas J.

In: Infection and Immunity, Vol. 77, No. 7, 07.2009, p. 2932-2942.

Research output: Contribution to journalArticle

Haneda, T, Winter, SE, Butler, BP, Wilson, RP, Tükel, Ç, Winter, MG, Godinez, I, Tsolis, RM & Bäumler, AJ 2009, 'The capsule-encoding viaB locus reduces intestinal inflammation by a Salmonella pathogenicity island 1-independent mechanism', Infection and Immunity, vol. 77, no. 7, pp. 2932-2942. https://doi.org/10.1128/IAI.00172-09
Haneda, Takeshi ; Winter, Sebastian E. ; Butler, Brian P. ; Wilson, R. Paul ; Tükel, Çagla ; Winter, Maria G. ; Godinez, Ivan ; Tsolis, Renée M. ; Bäumler, Andreas J. / The capsule-encoding viaB locus reduces intestinal inflammation by a Salmonella pathogenicity island 1-independent mechanism. In: Infection and Immunity. 2009 ; Vol. 77, No. 7. pp. 2932-2942.
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