The capsule-encoding viaB locus reduces intestinal inflammation by a Salmonella pathogenicity island 1-independent mechanism

Takeshi Haneda, Sebastian E. Winter, Brian P. Butler, R. Paul Wilson, Çagla Tükel, Maria G. Winter, Ivan Godinez, Renée M. Tsolis, Andreas J. Bäumler

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Salmonella enterica serotype Typhimurium elicits acute neutrophil influx in the human intestinal mucosa within 1 or 2 days after infection, resulting in inflammatory diarrhea. In contrast, no overt symptoms are observed within the first 1 or 2 weeks after infection with S. enterica serotype Typhi. Here we show that introduction of the capsule-encoding viaB locus of serotype Typhi reduced the ability of serotype Typhimurium to elicit acute intestinal inflammation in a streptomycin-pretreated mouse model. Serotype Typhimurium requires a functional invasion-associated type III secretion system (type III secretion system 1 [T3SS-1]) to elicit cecal inflammation within 48 h after infection of streptomycin-pretreated mice, and the presence of the viaB locus reduced its invasiveness for human intestinal epithelial cells in vitro. However, a reduced activity of T3SS-1 could not account for the ability of the viaB locus to attenuate cecal inflammation, because introduction of the viaB locus into an invasion-deficient serotype Typhimurium strain (invA mutant) resulted in a significant reduction of pathology and inflammatory cytokine expression in the cecum 5 days after infection of mice. We conclude that a T3SS-1-independent mechanism contributes to the ability of the viaB locus to reduce intestinal inflammation.

Original languageEnglish (US)
Pages (from-to)2932-2942
Number of pages11
JournalInfection and immunity
Volume77
Issue number7
DOIs
StatePublished - Jul 1 2009

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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    Haneda, T., Winter, S. E., Butler, B. P., Wilson, R. P., Tükel, Ç., Winter, M. G., Godinez, I., Tsolis, R. M., & Bäumler, A. J. (2009). The capsule-encoding viaB locus reduces intestinal inflammation by a Salmonella pathogenicity island 1-independent mechanism. Infection and immunity, 77(7), 2932-2942. https://doi.org/10.1128/IAI.00172-09