The cellular basis of corneal transparency: Evidence for 'corneal crystallins'

James V. Jester, Torben Moller-Pedersen, Jiying Huang, Christina M. Sax, Wm Todd Kays, H. Dwight Cavangh, W. Matthew Petroll, Joram Piatigorsky

Research output: Contribution to journalArticle

351 Scopus citations

Abstract

In vivo corneal light scattering measurements using a novel confocal microscope demonstrated greatly increased backscatter from corneal stromal fibrocytes (keratocytes) in opaque compared to transparent corneal tissue in both humans and rabbits. Additionally, two water-soluble proteins, transketolase (TKT) and aldehyde dehydrogenase class 1 (ALDH1), isolated from rabbit keratocytes showed unexpectedly abundant expression (~30% of the soluble protein) in transparent corneas and markedly reduced levels in opaque scleral fibroblasts or keratocytes from hazy, freeze injured regions of the cornea. Together these data suggest that the relatively high expressions of TKT and ALDH1 contribute to corneal transparency in the rabbit at the cellular level, reminiscent of enzyme-crystallins in the lens. We also note that ALDH1 accumulates in the rabbit corneal epithelial cells, rather than ALDH3 as seen in other mammals, consistent with the taxon-specificity observed among lens enzyme-crystallins. Our results suggest that corneal cells, like lens cells, may preferentially express water-soluble proteins, often enzymes, for controlling their optical properties.

Original languageEnglish (US)
Pages (from-to)613-622
Number of pages10
JournalJournal of cell science
Volume112
Issue number5
StatePublished - 1999

Keywords

  • Aldehyde dehydrogenase class 1
  • Corneal transparency
  • Crystallin
  • Keratocyte
  • Transketolase

ASJC Scopus subject areas

  • Cell Biology

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  • Cite this

    Jester, J. V., Moller-Pedersen, T., Huang, J., Sax, C. M., Kays, W. T., Cavangh, H. D., Petroll, W. M., & Piatigorsky, J. (1999). The cellular basis of corneal transparency: Evidence for 'corneal crystallins'. Journal of cell science, 112(5), 613-622.