The chronically instrumented ewe. A model for studying vascular reactivity to angiotensin II in pregnancy

C. R. Rosenfeld, N. F. ;Gant

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

Vascular refractoriness to the systemic pressor effects of angiotensin II (AII) develops normally during human pregnancy. To ascertain if the ewe might provide a suitable animal model to study the mechanisms responsible for this response (unique to pregnancy) we studied this phenomenon in unanesthetized, chronically instrumented nonpregnant and pregnant sheep, 68-143 d gestation. In these studies dose-response curves were established for changes in both mean arterial pressure and uterine blood flow. The pressor response to continuous infusions of AII increases as a function of the dose of AII in both nonpregnant and pregnant animals (P<0.001), R = 0.943 and 0.879, respectively. However, the pregnant animals were refractory to the pressor effects of AII, requiring 0.016 μg of AII/min per kg to elicit a 20 mm Hg rise in mean arterial pressure, in contrast to 0.009 for nonpregnant animals. The slope and intercept for the regression lines are different at P<0.001. In pregnant animals the dose-response curve for uterine blood blow was also determined. Increases in uterine blood flow were observed at doses of AII <0.016 μg/min per kg, while larger doses resulted in a progressively greater reduction in blood flow. It appears likely that the ewe may serve as an animal model suitable for the further study of the unique pregnancy-modified systemic and uteroplacental vascular responses elicited by AII.

Original languageEnglish (US)
Pages (from-to)486-492
Number of pages7
JournalJournal of Clinical Investigation
Volume67
Issue number2
DOIs
StatePublished - Jan 1 1981

ASJC Scopus subject areas

  • Medicine(all)

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