TY - JOUR
T1 - The circadian clock-containing photoreceptor cells in Xenopus laevis express several isoforms of casein kinase I
AU - Constance, Cara M.
AU - Fan, Jin Yuan
AU - Preuss, Fabian
AU - Green, Carla B.
AU - Price, Jeffrey L.
N1 - Funding Information:
We thank Ed Bjes and Silvia LaRue for excellent technical assistance and J.M. Graff for the full-length CKIε clones used in this study. The work was supported by Public Health Services grants from the National Institutes of Health to J.L.P (R01 MH056895) and to C.B.G. (R01 MH61461), a grant from the University of Missouri Research Board to J.L.P., and a Fight for Sight Postdoctoral Fellowship to C.C.
PY - 2005/5/20
Y1 - 2005/5/20
N2 - The frog (Xenopus laevis) retina has been an important model for the analysis of retinal circadian rhythms. In this paper, several isoforms of X. laevis casein kinase I (CKI) were analyzed to address whether they are involved in the phosphorylation and degradation of period protein (PER), as they are in the circadian oscillators of other species. cDNAs encoding two splice variants of CKIδ (a full-length form and deletion isoform, which is missing an exon that encodes a putative nuclear localization signal and two evolutionarily conserved protein kinase domains) were isolated and analyzed, together with a previously isolated CKIε isoform. Both CKIδ and CKIε were shown to be constitutively expressed in the photoreceptors of the retina, where a circadian clock has been localized. Both the full-length CKIδ and CKIε were shown to have kinase activity in vitro, and the full-length CKIδ phosphorylated and degraded Drosophila PER when expressed in Drosophila S2 cells. The expression and biochemical characteristics of these CKIs are consistent with an evolutionarily conserved role for CKI in the Xenopus retinal clock. The CKIδ deletion isoform did not exhibit kinase activity and did not trigger degradation of PER. Subcellular localization of both CKIδ isoforms was cytoplasmic in several cell culture lines, but the full-length CKIδ, and not the deletion CKIδ isoform, was localized to both the nucleus and the cytoplasm in Drosophila S2 cells. These results indicate that the sequences missing in the deletion CKIδ isoform are important for the nuclear localization and kinase activity of the full-length isoform and that one or both of these features are necessary for degradation of Drosophila PER.
AB - The frog (Xenopus laevis) retina has been an important model for the analysis of retinal circadian rhythms. In this paper, several isoforms of X. laevis casein kinase I (CKI) were analyzed to address whether they are involved in the phosphorylation and degradation of period protein (PER), as they are in the circadian oscillators of other species. cDNAs encoding two splice variants of CKIδ (a full-length form and deletion isoform, which is missing an exon that encodes a putative nuclear localization signal and two evolutionarily conserved protein kinase domains) were isolated and analyzed, together with a previously isolated CKIε isoform. Both CKIδ and CKIε were shown to be constitutively expressed in the photoreceptors of the retina, where a circadian clock has been localized. Both the full-length CKIδ and CKIε were shown to have kinase activity in vitro, and the full-length CKIδ phosphorylated and degraded Drosophila PER when expressed in Drosophila S2 cells. The expression and biochemical characteristics of these CKIs are consistent with an evolutionarily conserved role for CKI in the Xenopus retinal clock. The CKIδ deletion isoform did not exhibit kinase activity and did not trigger degradation of PER. Subcellular localization of both CKIδ isoforms was cytoplasmic in several cell culture lines, but the full-length CKIδ, and not the deletion CKIδ isoform, was localized to both the nucleus and the cytoplasm in Drosophila S2 cells. These results indicate that the sequences missing in the deletion CKIδ isoform are important for the nuclear localization and kinase activity of the full-length isoform and that one or both of these features are necessary for degradation of Drosophila PER.
KW - Alternative splicing
KW - Doubletime
KW - Nuclear localization
KW - Period protein
KW - Protein phosphorylation
KW - Protein stability
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U2 - 10.1016/j.molbrainres.2005.02.009
DO - 10.1016/j.molbrainres.2005.02.009
M3 - Article
C2 - 15893604
AN - SCOPUS:18944389537
SN - 0169-328X
VL - 136
SP - 199
EP - 211
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1-2
ER -