Abstract
Fine-tuning of transcriptional responses can be critical for long-term outcomes in response to an environmental challenge. The circadian protein Nocturnin belongs to a family of proteins that include exonucleases, endonucleases, and phosphatases and is most closely related to the CCR4 family of deadenylases that regulate the cellular transcriptome via control of poly(A) tail length of RNA transcripts. In this study, we investigate the role of Nocturnin in regulating the transcriptional response and downstream metabolic adaptations during cold exposure in brown adipose tissue. We find that Nocturnin exhibits dual localization within the cytosol and mitochondria, and loss of Nocturnin causes changes in expression of networks of mRNAs involved in mitochondrial function. Furthermore, Nocturnin−/− animals display significantly elevated levels of tricarboxylic acid cycle intermediates, indicating that they have distinct metabolic adaptations during a prolonged cold exposure. We conclude that cold-induced stimulation of Nocturnin levels can regulate long-term metabolic adaptations to environmental challenges. Biological Sciences; Cell Biology; Metabolomics; Transcriptomics.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 83-92 |
| Number of pages | 10 |
| Journal | iScience |
| Volume | 19 |
| DOIs | |
| State | Published - Sep 27 2019 |
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Keywords
- Biological Sciences
- Cell Biology
- Metabolomics
- Transcriptomics
ASJC Scopus subject areas
- General
Cite this
The Circadian Protein Nocturnin Regulates Metabolic Adaptation in Brown Adipose Tissue. / Onder, Yasemin; Laothamatas, I.; Berto, Stefano; Sewart, Katharina; Kilaru, Gokhul; Bordieanu, Bogdan; Stubblefield, Jeremy J.; Konopka, Genevieve; Mishra, Prashant; Green, Carla B.
In: iScience, Vol. 19, 27.09.2019, p. 83-92.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The Circadian Protein Nocturnin Regulates Metabolic Adaptation in Brown Adipose Tissue
AU - Onder, Yasemin
AU - Laothamatas, I.
AU - Berto, Stefano
AU - Sewart, Katharina
AU - Kilaru, Gokhul
AU - Bordieanu, Bogdan
AU - Stubblefield, Jeremy J.
AU - Konopka, Genevieve
AU - Mishra, Prashant
AU - Green, Carla B
PY - 2019/9/27
Y1 - 2019/9/27
N2 - Fine-tuning of transcriptional responses can be critical for long-term outcomes in response to an environmental challenge. The circadian protein Nocturnin belongs to a family of proteins that include exonucleases, endonucleases, and phosphatases and is most closely related to the CCR4 family of deadenylases that regulate the cellular transcriptome via control of poly(A) tail length of RNA transcripts. In this study, we investigate the role of Nocturnin in regulating the transcriptional response and downstream metabolic adaptations during cold exposure in brown adipose tissue. We find that Nocturnin exhibits dual localization within the cytosol and mitochondria, and loss of Nocturnin causes changes in expression of networks of mRNAs involved in mitochondrial function. Furthermore, Nocturnin−/− animals display significantly elevated levels of tricarboxylic acid cycle intermediates, indicating that they have distinct metabolic adaptations during a prolonged cold exposure. We conclude that cold-induced stimulation of Nocturnin levels can regulate long-term metabolic adaptations to environmental challenges. Biological Sciences; Cell Biology; Metabolomics; Transcriptomics.
AB - Fine-tuning of transcriptional responses can be critical for long-term outcomes in response to an environmental challenge. The circadian protein Nocturnin belongs to a family of proteins that include exonucleases, endonucleases, and phosphatases and is most closely related to the CCR4 family of deadenylases that regulate the cellular transcriptome via control of poly(A) tail length of RNA transcripts. In this study, we investigate the role of Nocturnin in regulating the transcriptional response and downstream metabolic adaptations during cold exposure in brown adipose tissue. We find that Nocturnin exhibits dual localization within the cytosol and mitochondria, and loss of Nocturnin causes changes in expression of networks of mRNAs involved in mitochondrial function. Furthermore, Nocturnin−/− animals display significantly elevated levels of tricarboxylic acid cycle intermediates, indicating that they have distinct metabolic adaptations during a prolonged cold exposure. We conclude that cold-induced stimulation of Nocturnin levels can regulate long-term metabolic adaptations to environmental challenges. Biological Sciences; Cell Biology; Metabolomics; Transcriptomics.
KW - Biological Sciences
KW - Cell Biology
KW - Metabolomics
KW - Transcriptomics
UR - http://www.scopus.com/inward/record.url?scp=85069710043&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85069710043&partnerID=8YFLogxK
U2 - 10.1016/j.isci.2019.07.016
DO - 10.1016/j.isci.2019.07.016
M3 - Article
C2 - 31357170
AN - SCOPUS:85069710043
VL - 19
SP - 83
EP - 92
JO - iScience
JF - iScience
SN - 2589-0042
ER -