The class i myosin MYO1D binds to lipid and protects against colitis

William McAlpine, Kuan Wen Wang, Jin Huk Choi, Miguel San Miguel, Sarah Grace McAlpine, Jamie Russell, Sara Ludwig, Xiaohong Li, Miao Tang, Xiaoming Zhan, Mihwa Choi, Tao Wang, Chun Hui Bu, Anne R. Murray, Eva Marie Y. Moresco, Emre E. Turer, Bruce Beutler

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Myosin ID (MYO1D) is a member of the class I myosin family. We screened 48,649 third generation (G3) germline mutant mice derived from N-ethyl-N-nitrosourea-mutagenized grandsires for intestinal homeostasis abnormalities after oral administration of dextran sodium sulfate (DSS). We found and validated mutations in Myo1d as a cause of increased susceptibility to DSS-induced colitis. MYO1D is produced in the intestinal epithelium, and the colitis phenotype is dependent on the nonhematopoietic compartment of the mouse. Moreover, MYO1D appears to couple cytoskeletal elements to lipid in an ATP-dependent manner. These findings demonstrate that MYO1D is needed to maintain epithelial integrity and protect against DSSinduced colitis.

Original languageEnglish (US)
Article numberdmm035923
JournalDMM Disease Models and Mechanisms
Volume11
Issue number9
DOIs
StatePublished - Sep 2018

Keywords

  • Dextran sodium sulfate
  • Inflammatory bowel disease
  • N-ethyl-N-nitrosourea

ASJC Scopus subject areas

  • Neuroscience (miscellaneous)
  • Medicine (miscellaneous)
  • Immunology and Microbiology (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    McAlpine, W., Wang, K. W., Choi, J. H., Miguel, M. S., McAlpine, S. G., Russell, J., Ludwig, S., Li, X., Tang, M., Zhan, X., Choi, M., Wang, T., Bu, C. H., Murray, A. R., Moresco, E. M. Y., Turer, E. E., & Beutler, B. (2018). The class i myosin MYO1D binds to lipid and protects against colitis. DMM Disease Models and Mechanisms, 11(9), [dmm035923]. https://doi.org/10.1242/dmm.035923