The clearance of glycoproteins in diabetic mice

Salvatore V. Pizzo, Mark A. Lehrman, Michael J. Imber, C. Earl Guthrow

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The clearances of N-acetyl-D-glucosaminyl-bovine serum albumin (GlcNAc-BSA), L-fucosyl-bovine serum albumin (Fuc-BSA) and asialoorosomucoid (ASOR) were evaluated in alloxan-induced diabetic mice and compared to non-diabetic litter mates. The clearances of Fuc-BSA and ASOR were identical in both groups. However, the clearance of GlcNAc-BSA was considerably slower in the diabetic animals. When GlcNAc-BSA was injected into normal mice in the presence of a blood glucose of 25 to 50 mM, competition was observed. No competition was seen with Fuc-BSA or D-galactosyl-BSA (Gal-BSA) in the presence of 50 mM glucose. In, vitro binding studies with purified receptor for GlcNAc/mannose confirm that glucose competes for binding of GlcNAc-BSA to this receptor.

Original languageEnglish (US)
Pages (from-to)704-708
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume101
Issue number2
DOIs
StatePublished - Jul 30 1981

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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