The cloning and expression of a sodium channel β1-subunit cDNA from human brain

Andrea I. McClatchey, Stephen C. Cannon, Susan A. Slaugenhaupt, James F. Gusella

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Electrical excitability of neurons and muscle cells is mediated largely through the actions of the voltage-gated sodium channel. Initiation and propagation of the action potential is a direct result of the precisely controlled inward flux of sodium through these channels. Much attention has been paid to the sodium channel α-subunit, the major, pore-forming component. However, α-subunits are associated with one or more smaller β-subunits, which have been implicated in the critical fine tuning of the gating properties of the channel. To investigate the properties of the β-subunit, we have isolated a cDNA encoding the human brain β1-subunit and assigned the corresponding gene to chromosome 19. We have also examined the effects of expressing the brain β1-subunit on the kinetics of a coexpressed muscle sodium channel α-subunit. Our results underscore the functional importance of the β1-subunit and imply a conserved mechanism for the interaction of the β1-subunit with different α-subunits.

Original languageEnglish (US)
Pages (from-to)745-749
Number of pages5
JournalHuman Molecular Genetics
Volume2
Issue number6
StatePublished - Jun 1993

Fingerprint

Sodium Channels
Cloning
CDNA
Sodium
Organism Cloning
Brain
Complementary DNA
Muscle
Voltage-Gated Sodium Channels
Chromosomes, Human, Pair 19
Excitability
Muscle Cells
Action Potentials
Action Potential
Chromosomes
Neurons
Chromosome
Neuron
Muscles
Tuning

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Public Health, Environmental and Occupational Health
  • Molecular Biology
  • Genetics(clinical)

Cite this

McClatchey, A. I., Cannon, S. C., Slaugenhaupt, S. A., & Gusella, J. F. (1993). The cloning and expression of a sodium channel β1-subunit cDNA from human brain. Human Molecular Genetics, 2(6), 745-749.

The cloning and expression of a sodium channel β1-subunit cDNA from human brain. / McClatchey, Andrea I.; Cannon, Stephen C.; Slaugenhaupt, Susan A.; Gusella, James F.

In: Human Molecular Genetics, Vol. 2, No. 6, 06.1993, p. 745-749.

Research output: Contribution to journalArticle

McClatchey, AI, Cannon, SC, Slaugenhaupt, SA & Gusella, JF 1993, 'The cloning and expression of a sodium channel β1-subunit cDNA from human brain', Human Molecular Genetics, vol. 2, no. 6, pp. 745-749.
McClatchey AI, Cannon SC, Slaugenhaupt SA, Gusella JF. The cloning and expression of a sodium channel β1-subunit cDNA from human brain. Human Molecular Genetics. 1993 Jun;2(6):745-749.
McClatchey, Andrea I. ; Cannon, Stephen C. ; Slaugenhaupt, Susan A. ; Gusella, James F. / The cloning and expression of a sodium channel β1-subunit cDNA from human brain. In: Human Molecular Genetics. 1993 ; Vol. 2, No. 6. pp. 745-749.
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