The common fragile site FRA16D and its associated gene WWOX are highly conserved in the mouse at Fra8E1

Kurt A. Krummel, Stacy R. Denison, Eric Calhoun, Leslie A. Phillips, David I. Smith

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Recently, several common fragile sites (CFSs) have been cloned and characterized, including the two most frequently observed in the human population, FRA3B and FRA16D. In addition to their high frequency of breakage, FRA3B and FRA16D colocalize with genes crossing large regions of breakage. At FRA3B, the fragile histidine triad (FHIT) gene spans more than 1 Mb, and at FRA16D, the WWOX gene spans more than 750 kb. It has also been shown that in Mus musculus a CFS Fra 14A2 and the mouse Fhit gene are conserved In the orthologous region of the genome. In this study, we positioned the ortholog to WWOX (Wox 1) at chromosome band 8E1 in the mouse genome. To determine whether, like Fra14A2 and Fhit, Fra8E1 and Wox1 colocalized in the mouse, we prepared bacterial and yeast artificial chromosome probes, and we hybridized them to aphidicolin-treated mouse metaphase chromosomes. Our data demonstrate that Wox1 colocalizes with Fra8E1. Furthermore, the sequence from this region, including introns, is highly conserved over at least a 100-kb region. This evolutionary conservation suggests that the two most active CFSs share many features, and that CFSs and their associated genes may be necessary for cell survival.

Original languageEnglish (US)
Pages (from-to)154-167
Number of pages14
JournalGenes Chromosomes and Cancer
Volume34
Issue number2
DOIs
StatePublished - 2002

Fingerprint

Genes
Genome
Yeast Artificial Chromosomes
Aphidicolin
Bacterial Artificial Chromosomes
Chromosomes, Human, Pair 1
Metaphase
Histidine
Introns
Cell Survival
Chromosomes
Population

ASJC Scopus subject areas

  • Cancer Research
  • Genetics

Cite this

The common fragile site FRA16D and its associated gene WWOX are highly conserved in the mouse at Fra8E1. / Krummel, Kurt A.; Denison, Stacy R.; Calhoun, Eric; Phillips, Leslie A.; Smith, David I.

In: Genes Chromosomes and Cancer, Vol. 34, No. 2, 2002, p. 154-167.

Research output: Contribution to journalArticle

Krummel, Kurt A. ; Denison, Stacy R. ; Calhoun, Eric ; Phillips, Leslie A. ; Smith, David I. / The common fragile site FRA16D and its associated gene WWOX are highly conserved in the mouse at Fra8E1. In: Genes Chromosomes and Cancer. 2002 ; Vol. 34, No. 2. pp. 154-167.
@article{f7b2b3bd1b6e4e34941ed2593f91ff11,
title = "The common fragile site FRA16D and its associated gene WWOX are highly conserved in the mouse at Fra8E1",
abstract = "Recently, several common fragile sites (CFSs) have been cloned and characterized, including the two most frequently observed in the human population, FRA3B and FRA16D. In addition to their high frequency of breakage, FRA3B and FRA16D colocalize with genes crossing large regions of breakage. At FRA3B, the fragile histidine triad (FHIT) gene spans more than 1 Mb, and at FRA16D, the WWOX gene spans more than 750 kb. It has also been shown that in Mus musculus a CFS Fra 14A2 and the mouse Fhit gene are conserved In the orthologous region of the genome. In this study, we positioned the ortholog to WWOX (Wox 1) at chromosome band 8E1 in the mouse genome. To determine whether, like Fra14A2 and Fhit, Fra8E1 and Wox1 colocalized in the mouse, we prepared bacterial and yeast artificial chromosome probes, and we hybridized them to aphidicolin-treated mouse metaphase chromosomes. Our data demonstrate that Wox1 colocalizes with Fra8E1. Furthermore, the sequence from this region, including introns, is highly conserved over at least a 100-kb region. This evolutionary conservation suggests that the two most active CFSs share many features, and that CFSs and their associated genes may be necessary for cell survival.",
author = "Krummel, {Kurt A.} and Denison, {Stacy R.} and Eric Calhoun and Phillips, {Leslie A.} and Smith, {David I.}",
year = "2002",
doi = "10.1002/gcc.10047",
language = "English (US)",
volume = "34",
pages = "154--167",
journal = "Genes Chromosomes and Cancer",
issn = "1045-2257",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - The common fragile site FRA16D and its associated gene WWOX are highly conserved in the mouse at Fra8E1

AU - Krummel, Kurt A.

AU - Denison, Stacy R.

AU - Calhoun, Eric

AU - Phillips, Leslie A.

AU - Smith, David I.

PY - 2002

Y1 - 2002

N2 - Recently, several common fragile sites (CFSs) have been cloned and characterized, including the two most frequently observed in the human population, FRA3B and FRA16D. In addition to their high frequency of breakage, FRA3B and FRA16D colocalize with genes crossing large regions of breakage. At FRA3B, the fragile histidine triad (FHIT) gene spans more than 1 Mb, and at FRA16D, the WWOX gene spans more than 750 kb. It has also been shown that in Mus musculus a CFS Fra 14A2 and the mouse Fhit gene are conserved In the orthologous region of the genome. In this study, we positioned the ortholog to WWOX (Wox 1) at chromosome band 8E1 in the mouse genome. To determine whether, like Fra14A2 and Fhit, Fra8E1 and Wox1 colocalized in the mouse, we prepared bacterial and yeast artificial chromosome probes, and we hybridized them to aphidicolin-treated mouse metaphase chromosomes. Our data demonstrate that Wox1 colocalizes with Fra8E1. Furthermore, the sequence from this region, including introns, is highly conserved over at least a 100-kb region. This evolutionary conservation suggests that the two most active CFSs share many features, and that CFSs and their associated genes may be necessary for cell survival.

AB - Recently, several common fragile sites (CFSs) have been cloned and characterized, including the two most frequently observed in the human population, FRA3B and FRA16D. In addition to their high frequency of breakage, FRA3B and FRA16D colocalize with genes crossing large regions of breakage. At FRA3B, the fragile histidine triad (FHIT) gene spans more than 1 Mb, and at FRA16D, the WWOX gene spans more than 750 kb. It has also been shown that in Mus musculus a CFS Fra 14A2 and the mouse Fhit gene are conserved In the orthologous region of the genome. In this study, we positioned the ortholog to WWOX (Wox 1) at chromosome band 8E1 in the mouse genome. To determine whether, like Fra14A2 and Fhit, Fra8E1 and Wox1 colocalized in the mouse, we prepared bacterial and yeast artificial chromosome probes, and we hybridized them to aphidicolin-treated mouse metaphase chromosomes. Our data demonstrate that Wox1 colocalizes with Fra8E1. Furthermore, the sequence from this region, including introns, is highly conserved over at least a 100-kb region. This evolutionary conservation suggests that the two most active CFSs share many features, and that CFSs and their associated genes may be necessary for cell survival.

UR - http://www.scopus.com/inward/record.url?scp=0036266065&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036266065&partnerID=8YFLogxK

U2 - 10.1002/gcc.10047

DO - 10.1002/gcc.10047

M3 - Article

C2 - 11979549

AN - SCOPUS:0036266065

VL - 34

SP - 154

EP - 167

JO - Genes Chromosomes and Cancer

JF - Genes Chromosomes and Cancer

SN - 1045-2257

IS - 2

ER -