The complete mitochondrial genome of Lerema accius and its phylogenetic implications

Qian Cong, Nick V. Grishin

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Butterflies and moths (Lepidoptera) are becoming model organisms for genetics and evolutionary biology. Decoding the Lepidoptera genomes, both nuclear and mitochondrial, is an essential step in these studies. Here we describe a protocol to assemble mitogenomes from Next Generation Sequencing reads obtained through whole-genome sequencing and report the 15,338 bp mitogenome of Lerema accius. The mitogenome is AT-rich and encodes 13 proteins, 22 transfer-RNAs, and two ribosomal- RNAs, with a gene order typical for Lepidoptera mitogenomes. A phylogenetic study based on the protein sequences using both Bayesian Inference and Maximum Likeli- hood methods consistently place Lerema accius with other grass skippers (Hesperiinae).

Original languageEnglish (US)
Article numbere1546
JournalPeerJ
Volume2016
Issue number1
DOIs
StatePublished - 2016

Keywords

  • Clouded Skipper
  • De novo assembly
  • Hesperiinae
  • Illumina sequencing

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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