The complete mitochondrial genome of Papilio glaucus and its phylogenetic implications

Jinhui Shen, Qian Cong, Nick V. Grishin

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Due to the intriguing morphology, lifecycle, and diversity of butterflies and moths, Lepidoptera are emerging as model organisms for the study of genetics, evolution and speciation. The progress of these studies relies on decoding Lepidoptera genomes, both nuclear and mitochondrial. Here we describe a protocol to obtain mitogenomes from Next Generation Sequencing reads performed for whole-genome sequencing and report the complete mitogenome of Papilio (Pterourus) glaucus. The circular mitogenome is 15,306. bp in length and rich in A and T. It contains 13 protein-coding genes (PCGs), 22 transfer-RNA-coding genes (tRNA), and 2 ribosomal-RNA-coding genes (rRNA), with a gene order typical for mitogenomes of Lepidoptera. We performed phylogenetic analyses based on PCG and RNA-coding genes or protein sequences using Bayesian Inference and Maximum Likelihood methods. The phylogenetic trees consistently show that among species with available mitogenomes Papilio glaucus is the closest to Papilio (Agehana) maraho from Asia.

Original languageEnglish (US)
Pages (from-to)68-83
Number of pages16
JournalMeta Gene
Volume5
DOIs
StatePublished - Sep 1 2015

Fingerprint

Lepidoptera
Mitochondrial Genome
Genetic Speciation
Genome
Butterflies
Proteins
Gene Order
Molecular Evolution
Moths
Transfer RNA
rRNA Genes
RNA
Genes

Keywords

  • Illumina sequencing
  • Mitochondrial genome
  • Papilio glaucus
  • Phylogeny

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

The complete mitochondrial genome of Papilio glaucus and its phylogenetic implications. / Shen, Jinhui; Cong, Qian; Grishin, Nick V.

In: Meta Gene, Vol. 5, 01.09.2015, p. 68-83.

Research output: Contribution to journalArticle

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