The conserved Ala37 in the ERF/AP2 domain is essential for binding with the DRE element and the GCC box

Yang Liu, Tong Jin Zhao, Jing Mei Liu, Wei Qun Liu, Qiang Liu, Yong Bin Yan, Hai Meng Zhou

Research output: Contribution to journalArticle

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Abstract

Four AP2/EREBP genes encoding putative ethylene-responsive element binding factor (ERF)/AP2 domains were cloned from Brassica napus, and these genes could be induced by low temperature, ethylene, drought, high salinity, abscisic acid and jasmonate treatments. These four genes, named BnDREBIII-1 to BnDREBIII-4, were highly homologous and the 37th amino acid was the only difference among their ERF/AP2 domains. BnDREBIII-1 was demonstrated to be able to bind to both dehydration-responsive element and the GCC box and transactivate the expression of downstream genes, while BnDREBIII-4 could bind neither. Further results suggested that Ala37 might play a crucial role in the DNA binding or the stability of the ERF/AP2 domain.

Original languageEnglish (US)
Pages (from-to)1303-1308
Number of pages6
JournalFEBS Letters
Volume580
Issue number5
DOIs
StatePublished - Feb 20 2006

Fingerprint

Genes
Brassica napus
Abscisic Acid
Gene encoding
Drought
Droughts
Salinity
Dehydration
Gene Expression
Amino Acids
Temperature
DNA
ethylene
jasmonic acid

Keywords

  • BnDREBIII
  • Brassica napus
  • Dehydration-responsive element
  • ERF/AP2 domain
  • GCC box

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Liu, Y., Zhao, T. J., Liu, J. M., Liu, W. Q., Liu, Q., Yan, Y. B., & Zhou, H. M. (2006). The conserved Ala37 in the ERF/AP2 domain is essential for binding with the DRE element and the GCC box. FEBS Letters, 580(5), 1303-1308. https://doi.org/10.1016/j.febslet.2006.01.048

The conserved Ala37 in the ERF/AP2 domain is essential for binding with the DRE element and the GCC box. / Liu, Yang; Zhao, Tong Jin; Liu, Jing Mei; Liu, Wei Qun; Liu, Qiang; Yan, Yong Bin; Zhou, Hai Meng.

In: FEBS Letters, Vol. 580, No. 5, 20.02.2006, p. 1303-1308.

Research output: Contribution to journalArticle

Liu, Y, Zhao, TJ, Liu, JM, Liu, WQ, Liu, Q, Yan, YB & Zhou, HM 2006, 'The conserved Ala37 in the ERF/AP2 domain is essential for binding with the DRE element and the GCC box', FEBS Letters, vol. 580, no. 5, pp. 1303-1308. https://doi.org/10.1016/j.febslet.2006.01.048
Liu, Yang ; Zhao, Tong Jin ; Liu, Jing Mei ; Liu, Wei Qun ; Liu, Qiang ; Yan, Yong Bin ; Zhou, Hai Meng. / The conserved Ala37 in the ERF/AP2 domain is essential for binding with the DRE element and the GCC box. In: FEBS Letters. 2006 ; Vol. 580, No. 5. pp. 1303-1308.
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abstract = "Four AP2/EREBP genes encoding putative ethylene-responsive element binding factor (ERF)/AP2 domains were cloned from Brassica napus, and these genes could be induced by low temperature, ethylene, drought, high salinity, abscisic acid and jasmonate treatments. These four genes, named BnDREBIII-1 to BnDREBIII-4, were highly homologous and the 37th amino acid was the only difference among their ERF/AP2 domains. BnDREBIII-1 was demonstrated to be able to bind to both dehydration-responsive element and the GCC box and transactivate the expression of downstream genes, while BnDREBIII-4 could bind neither. Further results suggested that Ala37 might play a crucial role in the DNA binding or the stability of the ERF/AP2 domain.",
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AU - Liu, Yang

AU - Zhao, Tong Jin

AU - Liu, Jing Mei

AU - Liu, Wei Qun

AU - Liu, Qiang

AU - Yan, Yong Bin

AU - Zhou, Hai Meng

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