The crystal structure of trp aporepressor at 1.8 Å shows how binding tryptophan enhances DNA affinity

R. G. Zhang, A. Joachimiak, C. L. Lawson, R. W. Schevitz, Z. Otwinowski, P. B. Sigler

Research output: Contribution to journalArticle

214 Citations (Scopus)

Abstract

Comparison of the crystal structure of inactive unliganded trp aporepressor with that of trp repressor shows that binding tryptophan activates the dimer a thousandfold by moving two symmetrically-disposed flexible bihelical motifs. These flexible 'DNA-reading heads' flank a highly inflexible core domain formed by an unusual arrangement of interlocking α-helices from both subunits.

Original languageEnglish (US)
Pages (from-to)591-597
Number of pages7
JournalNature
Volume327
Issue number6123
StatePublished - 1987

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Tryptophan
Reading
Head
DNA
E coli TRPR protein

ASJC Scopus subject areas

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Zhang, R. G., Joachimiak, A., Lawson, C. L., Schevitz, R. W., Otwinowski, Z., & Sigler, P. B. (1987). The crystal structure of trp aporepressor at 1.8 Å shows how binding tryptophan enhances DNA affinity. Nature, 327(6123), 591-597.

The crystal structure of trp aporepressor at 1.8 Å shows how binding tryptophan enhances DNA affinity. / Zhang, R. G.; Joachimiak, A.; Lawson, C. L.; Schevitz, R. W.; Otwinowski, Z.; Sigler, P. B.

In: Nature, Vol. 327, No. 6123, 1987, p. 591-597.

Research output: Contribution to journalArticle

Zhang, RG, Joachimiak, A, Lawson, CL, Schevitz, RW, Otwinowski, Z & Sigler, PB 1987, 'The crystal structure of trp aporepressor at 1.8 Å shows how binding tryptophan enhances DNA affinity', Nature, vol. 327, no. 6123, pp. 591-597.
Zhang RG, Joachimiak A, Lawson CL, Schevitz RW, Otwinowski Z, Sigler PB. The crystal structure of trp aporepressor at 1.8 Å shows how binding tryptophan enhances DNA affinity. Nature. 1987;327(6123):591-597.
Zhang, R. G. ; Joachimiak, A. ; Lawson, C. L. ; Schevitz, R. W. ; Otwinowski, Z. ; Sigler, P. B. / The crystal structure of trp aporepressor at 1.8 Å shows how binding tryptophan enhances DNA affinity. In: Nature. 1987 ; Vol. 327, No. 6123. pp. 591-597.
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