The cytotoxic type 3 secretion system 1 of Vibrio rewires host gene expression to subvert cell death and activate cell survival pathways

Nicole J. De Nisco, Mohammed Kanchwala, Peng Li, Jessie Fernandez, Chao Xing, Kim Orth

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Bacterial effectors potently manipulate host signaling pathways. The marine bacterium Vibrio parahaemolyticus (V. para) delivers effectors into host cells through two type 3 secretion systems (T3SSs). T3SS1 is vital for V. para survival in the environment, whereas T3SS2 causes acute gastroenteritis in human hosts. Although the natural host is undefined, T3SS1 effectors attack highly conserved cellular processes and pathways to orchestrate nonapoptotic cell death. To understand how the concerted action of T3SS1 effectors globally affects host cell signaling, we compared gene expression changes over time in primary fibroblasts infected with V. para that have a functional T3SS1 (T3SS1+) to those in cells infected with V. para lacking T3SS1 (T3SS1). Overall, the host transcriptional response to both T3SS1+and T3SS1 V. para was rapid, robust, and temporally dynamic. T3SS1 rewired host gene expression by specifically altering the expression of 398 genes. Although T3SS1 effectors targeted host cells at the posttranslational level to cause cytotoxicity, V. para T3SS1 also precipitated a host transcriptional response that initially activated cell survival and repressed cell death networks. The increased expression of several key prosurvival transcripts mediated by T3SS1 depended on a host signaling pathway that is silenced posttranslationally later in infection. Together, our analysis reveals a complex interplay between the roles of T3SS1 as both a transcriptional and posttranslational manipulator of host cell signaling.

Original languageEnglish (US)
Article numberl4501
JournalScience Signaling
Volume10
Issue number479
DOIs
StatePublished - May 16 2017

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Cell signaling
Vibrio parahaemolyticus
Vibrio
Cell death
Gene expression
Cell Survival
Cell Death
Cells
Gene Expression
Fibroblasts
Cytotoxicity
Manipulators
Bacteria
Genes
Gastroenteritis
Type III Secretion Systems
Survival
Infection

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

The cytotoxic type 3 secretion system 1 of Vibrio rewires host gene expression to subvert cell death and activate cell survival pathways. / De Nisco, Nicole J.; Kanchwala, Mohammed; Li, Peng; Fernandez, Jessie; Xing, Chao; Orth, Kim.

In: Science Signaling, Vol. 10, No. 479, l4501, 16.05.2017.

Research output: Contribution to journalArticle

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