The damage-responsive Drosophila gene sickle encodes a novel IAP binding protein similar to but distinct from Reaper, Grim, and Hid

Anna Christich, Saila Kauppila, Po Chen, Naoko Sogame, Su Inn Ho, John M. Abrams

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

In flies and mammals, critical regulators of cell death function by antagonizing Inhibitor of Apoptosis Proteins (IAPs), which themselves directly block caspase action [1]. The three currently known IAP antagonists in Drosophila map to the H99 genomic interval required for all programmed cell death [2]. Here we describe a fourth member of this genetic group, sickle (skl), which maps just outside of the H99 deletion. At its N terminus, Skl shares residues in common with other IAP antagonists in flies (Rpr, Grim, and Hid) [3-5] and in mammals (Smac/DIABLO and Omi/Htra2) [6-10]. Like other activators of apoptosis mapping in the Reaper region, full-length skl induced apoptosis when overexpressed, and the N terminus of this protein specifically bound to the BIR2 domain of DIAP1. However, unlike the N termini of Grim, Hid [11, 12], and Rpr, the N terminus of Skl did not induce apoptosis, skl transcripts accumulate in cells that are fated to die in some but not all regions of the embryo. Genotoxic stimuli induced skl expression, but skl was not responsive to all signals that trigger premature apoptosis, skl is potentially a fourth IAP antagonist in the "Reaper region" and a new candidate transducer of apoptotic damage signaling in Drosophila.

Original languageEnglish (US)
Pages (from-to)137-140
Number of pages4
JournalCurrent Biology
Volume12
Issue number2
DOIs
StatePublished - Jan 22 2002

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Inhibitor of Apoptosis Proteins
hides and skins
protein binding
Protein Binding
Drosophila
binding proteins
Carrier Proteins
apoptosis
Genes
Apoptosis
Mammals
Cell death
Diptera
Cell Death
genes
Caspase 1
antagonists
Caspases
Transducers
proteins

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

The damage-responsive Drosophila gene sickle encodes a novel IAP binding protein similar to but distinct from Reaper, Grim, and Hid. / Christich, Anna; Kauppila, Saila; Chen, Po; Sogame, Naoko; Ho, Su Inn; Abrams, John M.

In: Current Biology, Vol. 12, No. 2, 22.01.2002, p. 137-140.

Research output: Contribution to journalArticle

Christich, Anna ; Kauppila, Saila ; Chen, Po ; Sogame, Naoko ; Ho, Su Inn ; Abrams, John M. / The damage-responsive Drosophila gene sickle encodes a novel IAP binding protein similar to but distinct from Reaper, Grim, and Hid. In: Current Biology. 2002 ; Vol. 12, No. 2. pp. 137-140.
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