TY - JOUR
T1 - The detection of differentially expressed microRNAs from the serum of ovarian cancer patients using a novel real-time PCR platform
AU - Resnick, Kimberly E.
AU - Alder, Hansjuerg
AU - Hagan, John P.
AU - Richardson, Debra L.
AU - Croce, Carlo M.
AU - Cohn, David E.
PY - 2009/1/1
Y1 - 2009/1/1
N2 - Objective: To determine the utility of serum miRNAs as biomarkers for epithelial ovarian cancer. Methods: Twenty-eight patients with histologically confirmed epithelial ovarian cancer were identified from a tissue and serum bank. Serum was collected prior to definitive therapy. Fifteen unmatched, healthy controls were used for comparison. Serum was obtained from all patients. RNA was extracted using a derivation of the single step Trizol method. The RNA from 9 cancer specimens was compared to 4 normal specimens with real-time PCR using the TaqMan Array Human MicroRNA panel. Twenty-one miRNAs were differentially expressed between normal and patient serum. Real-time PCR for the 21 individual miRNAs was performed on the remaining 19 cancer specimens and 11 normal specimens. Results: Eight miRNAs of the original twenty-one were identified that were significantly differentially expressed between cancer and normal specimens using the comparative Ct method. MiRNAs-21, 92, 93, 126 and 29a were significantly over-expressed in the serum from cancer patients compared to controls (p < .01). MiRNAs-155, 127 and 99b were significantly under-expressed (p < .01). Additionally, miRs-21, 92 and 93 were over-expressed in 3 patients with normal pre-operative CA-125. Conclusion: We demonstrate that the extraction of RNA and subsequent identification of miRNAs from the serum of individuals diagnosed with ovarian cancer is feasible. Real-time PCR-based microarray is a novel and practical means to performing high-throughput investigation of serum RNA samples. miRNAs-21, 92 and 93 are known oncogenes with therapeutic and biomarker potential.
AB - Objective: To determine the utility of serum miRNAs as biomarkers for epithelial ovarian cancer. Methods: Twenty-eight patients with histologically confirmed epithelial ovarian cancer were identified from a tissue and serum bank. Serum was collected prior to definitive therapy. Fifteen unmatched, healthy controls were used for comparison. Serum was obtained from all patients. RNA was extracted using a derivation of the single step Trizol method. The RNA from 9 cancer specimens was compared to 4 normal specimens with real-time PCR using the TaqMan Array Human MicroRNA panel. Twenty-one miRNAs were differentially expressed between normal and patient serum. Real-time PCR for the 21 individual miRNAs was performed on the remaining 19 cancer specimens and 11 normal specimens. Results: Eight miRNAs of the original twenty-one were identified that were significantly differentially expressed between cancer and normal specimens using the comparative Ct method. MiRNAs-21, 92, 93, 126 and 29a were significantly over-expressed in the serum from cancer patients compared to controls (p < .01). MiRNAs-155, 127 and 99b were significantly under-expressed (p < .01). Additionally, miRs-21, 92 and 93 were over-expressed in 3 patients with normal pre-operative CA-125. Conclusion: We demonstrate that the extraction of RNA and subsequent identification of miRNAs from the serum of individuals diagnosed with ovarian cancer is feasible. Real-time PCR-based microarray is a novel and practical means to performing high-throughput investigation of serum RNA samples. miRNAs-21, 92 and 93 are known oncogenes with therapeutic and biomarker potential.
KW - Biomarkers
KW - MicroRNA
KW - Ovarian cancer
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U2 - 10.1016/j.ygyno.2008.08.036
DO - 10.1016/j.ygyno.2008.08.036
M3 - Article
C2 - 18954897
AN - SCOPUS:57649091633
VL - 112
SP - 55
EP - 59
JO - Gynecologic Oncology
JF - Gynecologic Oncology
SN - 0090-8258
IS - 1
ER -