The different roles of myosin IIA and myosin IIB in contraction of 3D collagen matrices by human fibroblasts

Zhenan Liu, Chin Han Ho, Frederick Grinnell

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Contraction of 3D collagen matrices by fibroblasts frequently is used as an in vitro model of wound closure. Different iterations of the model - all conventionally referred to as "contraction" - involve different morphological patterns. During floating matrix contraction, cells initially are round without stress fibers and subsequently undergo spreading. During stressed matrix contraction, cells initially are spread with stress fibers and subsequently undergo shortening. In the current studies, we used siRNA silencing of myosin IIA (MyoIIA) and myosin IIB (MyoIIB) to test the roles of myosin II isoforms in fibroblast interactions with 3D collagen matrices and collagen matrix contraction. We found that MyoIIA but not MyoIIB was required for cellular global inward contractile force, formation of actin stress fibers, and morphogenic cell clustering. Stressed matrix contraction required MyoIIA but not MyoIIB. Either MyoIIA or MyoIIB was sufficient for floating matrix contraction (FMC) stimulated by platelet-derived growth factor. Neither MyoIIA or MyoIIB was necessary for FMC stimulated by serum. Our findings suggest that myosin II-dependent motor mechanisms for collagen translocation during extracellular matrix remodeling differ depending on cell tension and growth factor stimulation.

Original languageEnglish (US)
Pages (from-to)295-306
Number of pages12
JournalExperimental Cell Research
Volume326
Issue number2
DOIs
StatePublished - Aug 15 2014

Fingerprint

Nonmuscle Myosin Type IIB
Nonmuscle Myosin Type IIA
Collagen
Fibroblasts
Stress Fibers
Myosin Type II
Platelet-Derived Growth Factor
Small Interfering RNA
Extracellular Matrix
Cluster Analysis
Actins
Intercellular Signaling Peptides and Proteins
Protein Isoforms
Wounds and Injuries
Serum

Keywords

  • 3D collagen matrix
  • Cell contraction
  • Morphogenic cell clustering
  • Myosin
  • Wound repair

ASJC Scopus subject areas

  • Cell Biology

Cite this

The different roles of myosin IIA and myosin IIB in contraction of 3D collagen matrices by human fibroblasts. / Liu, Zhenan; Ho, Chin Han; Grinnell, Frederick.

In: Experimental Cell Research, Vol. 326, No. 2, 15.08.2014, p. 295-306.

Research output: Contribution to journalArticle

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