The DNA binding domain of the rat liver nuclear protein C/EBP is bipartite

William H. Landschulz, Peter F. Johnson, Steven L. McKnight

Research output: Contribution to journalArticle

403 Citations (Scopus)

Abstract

C/EBP is a rat liver nuclear protein capable of sequence-specific interaction with DNA. The DNA sequences to which C/EBP binds in vitro have been implicated in the control of messenger RNA synthesis. It has therefore been predicted that C/EBP will play a role in regulating gene expression in mammalian cells. The region of the C/EBP polypeptide required for direct interaction with DNA has been identified and shown to bear amino acid sequence relatedness with the product of the myc, fos, and jun proto-oncogenes. The arrangement of these related amino acid sequences led to the prediction of a new structural motif, termed the "leucine zipper," that plays a role in facilitating sequence-specific interaction between protein and DNA. Experimental tests now provide support for the leucine zipper hypothesis.

Original languageEnglish (US)
Pages (from-to)1681-1688
Number of pages8
JournalScience
Volume243
Issue number4899
StatePublished - 1989

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CCAAT-Enhancer-Binding Proteins
Leucine Zippers
Amino Acid Sequence
Liver
DNA
jun Genes
Nuclear Proteins
Gene Expression
Messenger RNA
Proteins

ASJC Scopus subject areas

  • General

Cite this

Landschulz, W. H., Johnson, P. F., & McKnight, S. L. (1989). The DNA binding domain of the rat liver nuclear protein C/EBP is bipartite. Science, 243(4899), 1681-1688.

The DNA binding domain of the rat liver nuclear protein C/EBP is bipartite. / Landschulz, William H.; Johnson, Peter F.; McKnight, Steven L.

In: Science, Vol. 243, No. 4899, 1989, p. 1681-1688.

Research output: Contribution to journalArticle

Landschulz, WH, Johnson, PF & McKnight, SL 1989, 'The DNA binding domain of the rat liver nuclear protein C/EBP is bipartite', Science, vol. 243, no. 4899, pp. 1681-1688.
Landschulz, William H. ; Johnson, Peter F. ; McKnight, Steven L. / The DNA binding domain of the rat liver nuclear protein C/EBP is bipartite. In: Science. 1989 ; Vol. 243, No. 4899. pp. 1681-1688.
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