Two Sprague-Dawley rats were given injections of H3 thymidine sufficient to label the DNA of 80% of the lymphoid cells. Immediately after the labeling, a series of 8 wounds were inflicted in the rats on 8 successive days so that the first 8 days of wound healing were represented by separate wounds. At the time each wound was inflicted, a control biopsy was excised from the skin at the wound's margin. On the ninth day all wounds were excised. All wounds and their control biopsies were examined microscopically and autoradiographically. Examination of the slides revealed an increase in the number of labeled round cells and in the number of labeled fibroblasts in the wounds as compared with the control skin. These findings indicate the possibility that the round cells of the chronic inflammatory reaction act as trephocytes to nourish and stimulate newly formed fibroblasts.
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Molecular Biology
- Clinical Biochemistry