The DNA-unwinding mechanism of the ring helicase of bacteriophage T7

Yong Joo Jeong, Mikhail K. Levin, Smita S. Patel

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Helicases are motor proteins that use the chemical energy of NTP hydrolysis to drive mechanical processes such as translocation and nucleic acid strand separation. Bacteriophage T7 helicase functions as a hexameric ring to drive the replication complex by separating the DNA strands during genome replication. Our studies show that T7 helicase unwinds DNA with a low processivity, and the results indicate that the low processivity is due to ring opening and helicase dissociating from the DNA during unwinding. We have measured the single-turnover kinetics of DNA unwinding and globally fit the data to a modified stepping model to obtain the unwinding parameters. The comparison of the unwinding properties of T7 helicase with its translocation properties on single-stranded (ss)DNA has provided insights into the mechanism of strand separation that is likely to be general for ring helicases. T7 helicase unwinds DNA with a rate of 15 bp/s, which is 9-fold slower than the translocation speed along ssDNA. T7 helicase is therefore primarily an ssDNA translocase that does not directly destabilize duplex DNA. We propose that T7 helicase achieves DNA unwinding by its ability to bind ssDNA because it translocates unidirectionally, excluding the complementary strand from its central channel. The results also imply that T7 helicase by itself is not an efficient helicase and most likely becomes proficient at unwinding when it is engaged in a replication complex.

Original languageEnglish (US)
Pages (from-to)7264-7269
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number19
DOIs
StatePublished - May 11 2004

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DNA Helicases
Bacteriophage T7
DNA
Mechanical Phenomena
Single-Stranded DNA
Nucleic Acids
Hydrolysis
Genome
Proteins

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

The DNA-unwinding mechanism of the ring helicase of bacteriophage T7. / Jeong, Yong Joo; Levin, Mikhail K.; Patel, Smita S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 19, 11.05.2004, p. 7264-7269.

Research output: Contribution to journalArticle

Jeong, Yong Joo ; Levin, Mikhail K. ; Patel, Smita S. / The DNA-unwinding mechanism of the ring helicase of bacteriophage T7. In: Proceedings of the National Academy of Sciences of the United States of America. 2004 ; Vol. 101, No. 19. pp. 7264-7269.
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