The Drosophila clock gene double-time encodes a protein closely related to human casein kinase Iε

Brian Kloss, Jeffrey L. Price, Lino Saez, Justin Blau, Adrian Rothenfluh, Cedric S. Wesley, Michael W. Young

Research output: Contribution to journalArticlepeer-review

567 Scopus citations

Abstract

The cloning of double-time (db(t)) is reported. DOUBLETIME protein (DBT) is most closely related to human casein kinase Iε. dbt(S) and dbt(L) mutations, which alter period length of Drosophila circadian rhythms, produce single amino acid changes in conserved regions of the predicted kinase. dbt(P) mutants, which eliminate rhythms of per and tim expression and constitutively overproduce hypophosphorylated PER proteins, abolish most dbt expression. dbt mRNA appears to be expressed in the same cell types as are per and tim and shows no evident oscillation in wild-type heads. DBT is capable of binding to PER in vitro and in Drosophila cells, suggesting that a physical association of PER and DBT regulates PER phosphorylation and accumulation in vivo.

Original languageEnglish (US)
Pages (from-to)97-107
Number of pages11
JournalCell
Volume94
Issue number1
DOIs
StatePublished - Jul 10 1998

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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