The dynactin complex enhances the speed of microtubule-dependent motions of adenovirus both towards and away from the nucleus

Martin F. Engelke, Christoph J. Burckhardt, Matthias K. Morf, Urs F. Greber

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Unlike transport vesicles or organelles, human adenovirus (HAdV) directly binds to the microtubule minus end-directed motor dynein for transport to the nucleus. The dynein cofactor dynactin enhances nuclear transport of HAdV and boosts infection. To determine if dynactin has a specific role in cytoplasmic trafficking of incoming HAdV on microtubules, we used live cell spinning disc confocal microscopy at 25 Hz acquisition frequency and automated tracking of single virus particles at 20-50 nm spatial resolution. Computational dissection by machine-learning algorithms extracted specific motion patterns of viral trajectories. We found that unperturbed cells supported two kinds of microtubule-dependent motions, directed motions (DM) and fast drifts (FD). DM had speeds of 0.2 to 2 μm/s and run lengths of 0.4 up to 7 μm, while FD were slower and less extensive at 0.02 to 0.4 μm/s and 0.05 to 2.5 μm. Dynactin interference by overexpression of p50/dynamitin or a coiled-coil domain of p150/Glued reduced the speeds and amounts of both center- and periphery-directed DM but not FD, and inhibited infection. These results indicate that dynactin enhances adenovirus infection by increasing the speed and efficiency of dynein-mediated virus motion to the nucleus, and, surprisingly, also supports a hereto unknown motor activity for virus transport to the cell periphery.

Original languageEnglish (US)
Pages (from-to)233-253
Number of pages21
JournalViruses
Volume3
Issue number3
DOIs
StatePublished - Mar 1 2011
Externally publishedYes

Fingerprint

Adenoviridae
Microtubules
Dyneins
Human Adenoviruses
Human Adenovirus Infections
Viruses
Adenoviridae Infections
Transport Vesicles
Cell Nucleus Active Transport
Confocal Microscopy
Virion
Organelles
Dynactin Complex
Dissection
Motor Activity
Infection

Keywords

  • Cytoplasmic transport
  • Cytoskeleton
  • Dynein
  • Infection
  • Motor
  • Virus motion

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

The dynactin complex enhances the speed of microtubule-dependent motions of adenovirus both towards and away from the nucleus. / Engelke, Martin F.; Burckhardt, Christoph J.; Morf, Matthias K.; Greber, Urs F.

In: Viruses, Vol. 3, No. 3, 01.03.2011, p. 233-253.

Research output: Contribution to journalArticle

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